气相色谱和气相色谱/质谱法检测生物样品中氧化乐果  

Detection of omethoate in biomaterial by GC and GC/MS

作　　者：鞠芳芳[1,2] 张喜轩[1] 孙体健[2] 尉志文[2] 贠克明[2] 王玉瑾[2] 

机构地区：[1]中国医科大学法医学院,沈阳010001 [2]山西医科大学法医学院,太原030001

出　　处：《中国卫生检验杂志》2009年第12期2729-2730,2785,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家十一五科技支撑计划项目(2007BAK26B05)

摘　　要：目的：建立生物样品中氧化乐果的气相色谱和气相色谱/质谱定性定量分析方法。方法：心血和肝组织样品经三氯醋酸沉淀蛋白后,二氯甲烷萃取,气相色谱/质谱联用法定性分析、气相色谱内标法定量检测生物样品中氧化乐果。结果：心血和肝组织中氧化乐果气相色谱检测的回归方程、线性检测范围、相关系数、回收率、最低检出浓度分别为Y=0.0895X＋0.0474、0.5～80μg/ml、0.9906、98.87±1.11%、0.02μg/ml（S/N=3）和Y=13.628X-2.6199、0.5～120μg/g、0.9938、99.58±3.47%、0.02μg/g（S/N=3）。结论：建立的生物样品中氧化乐果的气相色谱/质谱检测方法选择性好,定性准确,气相色谱定量分析结果准确,可用于氧化乐果中毒的临床快速检验和氧化乐果中毒死亡案件的法医学鉴定。Objective：To establish a method for detecting the omethoate in biomaterial by GC and GC/MS.Methods：After precipitating protein in liver with trichloroacetic acid,omethoate in liver and blood was extracted with dichloromethane and analyzed qualitatively and quantitatively by GC/MS and GC,respectively.Results：The regression equation,linear range,correlation coefficient,recovery rate,limit of detection of omethoate in heart blood was Y=0.0895X＋0.0474,0.5~80 μg/ml,0.9906,98.87±1.11%,0.02 μg/ml and in liver was Y=13.628X-2.6199,0.5~80 μg/g,0.9938,99.58±3.47%,0.02 μg/g,respectively.Conclusion：The GC and GC/MS method for detecting omethoate in biomaterial is selective,accurate and could be applied not only to clinical diagnosis of omethoate poisoning but also to forensic identification of omethoate poisoning death.

关 键 词：氧化乐果 气相色谱法 气相色谱/质谱法 生物样品 

分 类 号：O657.71[理学—分析化学]