气相色谱法测定血清中碘的方法  

Determination of iodine in serum by gas chromatography

作　　者：张念华[1] 应英[1] 汤鋆[1] 宋国良[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2009年第12期2805-2806,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立气相色谱测定血清中碘的方法。方法：不同的衍生条件中,血清中碘和丁酮衍生成碘丁酮,再经HP-5（30 m×0.25 mm×0.25μm）毛细管柱分离,气相色谱电子捕获检测器检测。结果：在酸性环境、亚硫酸钠及3.5%双氧水衍生条件下,97%的碘酸盐中的碘转化为单质碘,与丁酮衍生成碘丁酮;该方法用于血清中碘的分析,测得血清中碘的浓度为780μg/L,碘的检出限为4μg/L,线性范围4～100μg/L,相关系数r=0.9992,加标回收率83.0%～95.0%,RSD为3.8%。结论：此方法能够用于血清中总碘含量的检测。Objective：To establish a method for determination of iodine in serum by gas chromatography.Methods：In different derivative matrix,iodine in serum was derived by butanone and the derivative was extracted by n-hexane.The derivative was separated by HP-5 capillary column（30 m×0.25 mm×0.25 μm） and detected by gas chromatography-electon capture detector.Results：In derivative matrix including acidification,sodium sulfite and 3.5% hydrogen peroxide,the iodate was derived by butanone,the derivative efficiency was 97%.The method was applied to analyze iodine,iodine in serum was 780 μg/L,the linear range was 4～100 μg/L.The correlation cofficiency was 0.9992,the recovery 83.0%～95.0%,RSD 3.8%.Conclusion：The method is suitable to analyze the iodine including iodate and iodide in serum.

关 键 词：碘 血清 气相色谱 

分 类 号：O657.71[理学—分析化学]