火焰原子吸收光谱法测定血清中不同化学形态锌  

Speciation analysis of trace elements Zn in serum by flame atomic absorvtion spectrophotometry

作　　者：刘雨[1] 杜维月 张哲[1] 

机构地区：[1]山东省聊城市疾病预防控制中心,山东聊城252000 [2]山东省阳谷县第一中学,山东阳谷252300

出　　处：《中国卫生检验杂志》2009年第12期2842-2843,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：融合分子生物学技术与原子吸收光谱对血清中元素锌的化学形态进行研究。方法：用60%乙醇低温（4℃）沉淀血清蛋白的方法将血清中的锌分为结合态和非结合态,原子吸收分光光度计分别测定血清中Zn的总量及非结合态含量,通过减差法求出血清中结合态元素含量。结果：该方法锌的检出限为1.06×10-3μg/ml,相对标准偏差为0.30%～0.96%,回收率为95.0%～106.0%。结论：建立了Zn元素的两种化学形态的分离分析方法,可用于实际应用。Objective：To study the chemical forms of Zn in the human serum by molecular biology techniques and flame atomic absorption spectrometry.Methods：The forms of combination and non-combination of Zn in the serum were separated by the 60% ethanol（v/v） on 4℃.The total and the non-combination concentrations of Zn in the serum were detected by flame atomic absorption spectrometry,then calculated the levels of combination of Zn.Results：The detection limit was 1.06×10-3 μg/ml with relative standard deviation less than 1.59%,and average recoveries varied from 95.0% to 106.0% in this method.

关 键 词：火焰原子吸收光谱法 锌 化学形态 

分 类 号：O657.31[理学—分析化学]