慢性乙型肝炎患者血清细胞因子检测的临床意义  

Clinical significance of determination of serum cytokine in patients with chronic hepatitis B

作　　者：周力[1] 王亮[1] 

机构地区：[1]吉林省人民医院,长春130021

出　　处：《中国卫生检验杂志》2009年第12期2866-2867,2924,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：了解慢性乙肝患者血清细胞因子变化与肝脏功能、HBV DNA含量的相关性。方法：选取2008年1月～2009年1月我院肝病科住院的慢性乙肝患者80例,并选取50例门诊体检者作为对照组,两组性别年龄无明显差异。所有研究对象均采静脉血5 ml,采用双抗体夹心ELISA方法,进行细胞因子：肿瘤坏死因子α（TNF-α）、γ干扰素（γ-IFN）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）水平测定和乙型肝炎标志物及肝功能分析;观察组同时用实时荧光定量PCR方法进行HBV DNA含量检测。结果：观察组为HbsAg和HbeAg阳性,ALT增高;血清HBV-DNA含量与ALT水平呈正相关（r=0.57,t=6.13）;慢性乙肝患者TNF-α、γ-IFN、IL-6、IL-10含量均较正常对照组高,其中TNF-α、IL-6、IL-10含量明显增高,与正常对照组比较差异显著,有统计学意义;慢性乙肝患者TNF-α、γ-IFN、IL-6、IL-10含量与ALT水平和HBV-DNA含量均呈正相关且有统计学意义。结论：慢性乙肝患者存在T淋巴细胞调控网络失衡。了解细胞因子在慢性乙肝发病中的作用,对于判断慢性乙肝病情及指导治...Objective：To explore serum cytokine changes and liver function,HBV DNA content of relevance in patients with chronic hepatitis B.Methods：Select 2008~2009 year in January inpatient hospital liver disease 80 cases of chronic hepatitis B patients,and 50 cases of out-patient physical examination were selected as the control group,no significant difference in sex and age groups.Have blood taken for all subjects 5ml,using double antibody sandwich ELISA method,the cell factor： tumor necrosis factor-α（TNF-α）,γ interferon（γ-IFN）,interleukin-6（IL-6）,Interleukin-10（IL-10） levels measured and hepatitis B markers and liver function analysis;the observation group at the same time using real-time fluorescence quantitative PCR method of detection of HBV DNA levels.Results：The observation group of HbsAg and HbeAg are positive,ALT increased;serum HBV-DNA levels and ALT levels were positively correlated（r=0.57,t=6.13,）;chronic hepatitis B patients with TNF-α,γ-IFN,IL-6,IL-10 levels higher than those in normal control group,in which TNF-α,IL-6,IL-10 were significantly higher compared with the normal control group,significant difference,there is statistical significance;chronic hepatitis B patients with TNF-α,γ-IFN,IL-6,IL-10 levels and ALT levels and HBV-DNA levels were positively correlated and there is statistically significant.Conclusion：Existence of T lymphocytes in patients with chronic hepatitis B regulatory networks imbalance.Understanding of cytokines in the pathogenesis of chronic hepatitis B,for judging the condition and guide the treatment of chronic hepatitis B are important.

关 键 词：慢性 乙型肝炎 细胞因子 

分 类 号：R519[医药卫生—内科学]