盐酸伊立替康脂质体包封率的测定  

Determination of entrapment efficiency of irinotecan liposomes

作　　者：陈超[1] 邓英杰[1] 王晓宇[1] 董晓东[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中国医药导报》2009年第36期68-70,共3页China Medical Herald

基　　金：国家"863"计划资助项目(2007AA021810)

摘　　要：目的:建立测定盐酸伊立替康脂质体包封率的方法。方法:通过微柱离心分离盐酸伊立替康脂质体和游离药物,以HPLC法测定药物含量,计算包封率。结果:Sephadex G-50微型柱可完全吸附盐酸伊立替康游离药物,空白脂质体回收率为98.2%～100.7%,脂质体和游离药物得到良好的分离;在选定色谱条件下,辅料不干扰测定,盐酸伊立替康质量浓度在1.1～10.6mg/L范围内线性关系良好(r=0.9997),日内和日间精密度(RSD)均<2%,加样回收率为97.6%～100.7%。结论:微柱离心-HPLC法可用于盐酸伊立替康脂质体包封率的测定。Objective：To establish a method for the determination of entrapment efficiency of irinotecan liposomes. Methods：Minicolumn centrifuge method was employed to separate the free drug from the liposomes. The content of irinotecan was quantified by HPLC. Results：Sephadex G-50 filling minicolumn could completely adsorb free irinotecan. The recovery of blank liposomes was in a range of 98.2%-100.7% by minicolumn centrifuge method. Irinotecan liposomes and free irinotecan could be well separated. A calibrated linear curve of irinotecan was within 1.1-10.6 mg/L （ r=0.999 7）. Intra-day and inter-day precision （RSD） were both less than 2%. The recovery of irinotecan with blank liposomes was from 97.6% to 100.7%. Conclusion：The minicolumn centrifuge-HPLC method can be used for the determination of entrapment efficiency of irinotecan liposomes.

关 键 词：盐酸伊立替康 脂质体 微柱离心 高效液相色谱法 包封率 

分 类 号：R927.2[医药卫生—药学]