复方通络胶囊对人脐静脉内皮细胞保护的作用机制  

The effect mechanism of Fufangtongluo Capsules on human umbilical vein endothelial cells injury

作　　者：王敬卿[1] 肖婧[2] 张绒[2] 

机构地区：[1]南京市中医院,江苏南京210001 [2]南京中医药大学,江苏南京210046

出　　处：《中国医药导报》2009年第36期90-91,共2页China Medical Herald

基　　金：南京市卫生局医学课题(YKK0456)

摘　　要：目的:观察复方通络胶囊对缺氧-复氧所致人脐静脉内皮细胞(HUVECs)损伤的作用机制。方法:体外培养HUVECs,建立缺氧-复氧模型,收集培养液,测定内皮素(ET)、一氧化氮(NO)、一氧化氮合酶(NOS)水平。结果:复方通络胶囊中、高剂量组能抑制缺氧-复氧诱导的ET释放,提高NOS的活性,促进NO的释放(P<0.05,P<0.01)。结论:复方通络胶囊通过抑制ET释放,提高NOS的活性,促进NO的释放,对人脐静脉内皮细胞缺氧-复氧损伤有明显的保护作用。Objective：To observe the mechanism of protective effect of Fufangtongluo Capsules on human umbilical vein endothelial cells （HUVECs） injury caused by hypoxia-reoxygenation. Methods：HUVECs were cultured in vitro to build the hypoxia-reoxygenation model,then we collected culture medium to determinate endothelin （ET）,nitric oxide （NO）,nitric oxide synthase （NOS） level. Results：We observed Fufangtongluo Capsules could inhibit the hypoxia-reoxygenation-induced ET release,improved NOS activity and promoted NO released in medium and high dose groups （P0.05,P0.01）. Conclusion：Fufangtongluo Capsules has obviously protective effect on human umbilical vein endothelial cells injury by inhibiting the release of ET,enhancing NOS activity and promoting the release of NO.

关 键 词：复方通络胶囊 内皮细胞 缺氧-复氧 作用机制 

分 类 号：R289[医药卫生—方剂学]