蛇毒神经生长因子诱导PC12细胞分化超微结构的观察  

Observation on differentiated ultrastructure behaviour induced by NGF in cu1tured PC12 cells

作　　者：覃柳燕[1] 张学荣[1] 班建东[1] 张钦乐[1] 赖允丽[1] 

机构地区：[1]广西医科大学蛇毒研究所,广西南宁530021

出　　处：《蛇志》2009年第4期257-260,共4页Journal of Snake

基　　金：广西自然科学基金(桂科能0630006-5E5Z;桂科能063006-5E4Z)

摘　　要：目的观察蛇毒神经生长因子(Nerve growth factor,NGF)诱导大鼠肾上腺嗜铬细胞瘤细胞系(Pheo-chromocytoma cells,PC12)细胞分化后,细胞超微结构的改变。方法取对数生长期PC12细胞接种24孔板,设200 ng/ml NGF实验组和对照组。培养72 h,离心,分别收集细胞制成电镜标本,镜下观察各组细胞超微结构的改变。结果与对照组细胞相比,实验组细胞长出大量突起,并且胞质的细胞器逐渐消失,出现较多的脂滴。结论广西眼镜蛇毒神经生长因子可以促进PC12细胞增殖,并诱导其向神经样细胞分化,长出突触。Objective Obseravated the changes of uttrastructure after differentiated induced by NGF in cultured PC12 cells. Methods Put PC12 cells that grew to logarithmic growth phase seeded into 24-well plates, set 200 ng/ml NGF experimental group and control group. After cultured 72 hours,we first centrifugated and then collected cells to make into electron microscope specimens,and observated the cells＇ ultrastructure changes happened in each group. Results Compared to control group,experimental group cells grew into a large number of protuberances,and organelles that are in cytoplasmic,and there were a lot of lipid droplets. Conclusion NGF can promote PC12 cells proliferation,and induced them differentiation into neural-like cells and grew synaptic.

关 键 词：神经生长因子 PC12细胞 超微结构 

分 类 号：R996.3[医药卫生—毒理学]