5种南海海藻醇提取物活性初步研究  

The bioactivity study of extractions from five algae collected from South China Sea

作　　者：史大永[1] 李敬[1] 郭书举[1] 苏华[1] 范晓[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071

出　　处：《海洋科学》2009年第12期40-43,共4页Marine Sciences

基　　金：国家863计划项目(2007AA09Z410)

摘　　要：海藻是海洋生物代谢产物的主要来源之一,能产生丰富多样的生物活性物质。为了更好地利用中国南海的海藻资源,分别对采自中国湛江硇洲岛的1种绿藻基根硬毛藻Chaetomorpha basiretorsa Setchell和4种红藻刺状鱼栖苔Acantophora spicififera(Vahl)Boerg、三列凹顶藻Laurencia tristicha Tseng,Chang etXia、紫杉状海门冬Asparagopsis taxiformis(Delile)Trevisan、鸡毛菜Pterocla diatenuis Okam进行了生物活性研究,分别对细胞毒活性(KB、Bel-7402、PC-3 M、Ketr3和MCF-7)、Na+,K+-ATPase抑制活性以及犬血管平滑肌细胞增殖抑制活性进行了筛选,部分海藻提取物表现出一定的生理活性。Algae, as favorite sources of marine secondary metabotites, contain rich kinds of bioactive compounds. The bioactive screen of ethanol extract of five marine algae, which were collected from Naozhou Island, the South Sea of China, was designed to search for bioactive compounds. The algae include one green alga Chaetomorpha basiretorsa Setchell and four red algae Acantophora spicififera （Vahl） Boerg, Laurencia tristicha Tseng, Chang et Xia, Asparagopsis taxiformis （Delile） Trevisan and Pterocladia tenuis Okam. A standard MTT method was used for testing cytotoxicity of ethanol extracts against human cancer cell lines KB, Bel-7402, PC-3M, Ketr3 and MCF-7. The inhibitory activity of extracts against Na^＋, K^＋- ATPase was carried out on an enzyme mode. The inhibitory activity of extracts against dog vascular smooth muscle cell proliferation was also screened by the MTT method. Some extracts showed moderate bioactivities.

关 键 词：海藻醇提取物 细胞毒活性 Na+ K+-AT Pase抑制活性 犬血管平滑肌细胞增殖抑制活性 

分 类 号：R282.77[医药卫生—中药学]