中药羚羊角及其类似品中水溶性蛋白的检测  

Detection on water soluble proteins of Cornu Saigae Tataricae and its analogues

作　　者：刘炎[1] 张贵君[1] 张杰[1] 石莹[2] 宋凤瑞[2] 刘志强[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国科学院长春应用化学研究所长春质谱中心,长春130022

出　　处：《中华中医药杂志》2009年第12期1571-1574,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：科技部基础性工作专项重点项目(No.2007FY130100)

摘　　要：目的:研究中药羚羊角及其类似品中水溶性蛋白组分差异及鉴定方法,为其药效组分质量评价体系的建立提供科学数据。方法:采用紫外分光光度法(UV)和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,对羚羊角及其类似品山羊角和黄羊角进行水溶性蛋白质含量和组分对比分析。结果:羚羊角中水溶性蛋白的含量高于黄羊角和山羊角,且3种角类中药蛋白组分的组成差异显著。结论:蛋白组分可用于羚羊角的鉴定,采用药效蛋白组分评价角类中药质量具有明显的科学性和实用性。Objective：To study the differences ofthe water soluble protein components between Cornu Saigae Tataricae and its analogues, cornu procaprae gutturosae and cornu caprae hircus, and the identification method.To provide the scientific date for developing the quality evaluation system ofactive components.Methods：Using UV spectrophotometry and sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） for analysis.Results：The water soluble protein content was higher in Cornu Saigae Tataricae than that in the other two samples, and the compositions ofpolyacrylamide gel electrophoretic bands showed significant difference between the three samples.Conclusion：The water soluble protein components could be used in the identification ofCornu Saigae Tataricae, it is scientific and practical to evaluate the quality ofmedicinal animal horns by using protein components alignment.

关 键 词：羚羊角 类似品 水溶性蛋白组分 药效组分质量评价 

分 类 号：R284.1[医药卫生—中药学]