香蕉枯萎病菌粗毒素对地衣芽胞杆菌生长和培养液上清蛋白组成的影响  

Effect of the Crude Toxin of Fusarium oxysporum f. sp. cubense Race 4 on the Growth and the Protein Secretion of Bacillus licheniforms

作　　者：黎永坚[1] 杨紫红[1] 陈远凤[1] 陈燕红[1] 程萍[1] 喻国辉[1] 

机构地区：[1]珠海市农业科学研究中心,广东珠海519075

出　　处：《微生物学通报》2009年第12期1826-1831,共6页Microbiology China

基　　金：广东省科技厅教育部产学研结合项目(No.2007B090400083);珠海市产学研结合项目(No.PC20082043);珠海市科技计划项目(No.PC20081078;PC20082043)

摘　　要：本文研究了香蕉枯萎病菌4号生理小种湛江菌株(Foc 4-zj)产生的粗毒素对地衣芽胞杆菌R21菌株生长及其培养液中蛋白组成变化的影响。实验结果表明,Foc 4-zj的粗毒素能够抑制R21菌株的生长,缩短其生长周期;减少培养液上清蛋白含量以及改变蛋白质的种类;低剂量的粗毒素有利于拮抗蛋白的积累,而高剂量的粗毒素则相反。The effects of the crude toxin of Fusarium oxysporum f. sp. cubense race 4 （Foc 4-zj） on the growth of Bacillus licheniforms R21 strain and its protein secretion were studied. The results showed that the growth of R21 strain was inhibited by the crude toxin produced by Foc 4-zj, and its growth cy- cle was shorten. The protein contents were decreased and the kinds of protein in the supernatant fluid had changed significantly after treated with crude toxin. High dose of the crude toxin greatly decreased the contents of antagonistic proteins, whereas lower one increased.

关 键 词：香蕉枯萎病菌4号生理小种-zj菌株 粗毒素 地衣芽胞杆菌R21菌株 生长曲线 拮抗蛋白 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]