冬虫夏草无性型菌丝体的原生质体诱变育种研究  

Breeding Cordyceps sinensis strains by protoplast mutagensis

作　　者：朱蕴兰[1] 陈安徽[1] 高淑云[1] 邵颖[1] 陈宏伟[1] 

机构地区：[1]徐州工程学院食品(生物)工程学院,徐州221008

出　　处：《食品科技》2009年第12期36-39,共4页Food Science and Technology

基　　金：江苏省高校自然科学研究计划项目(05KJD180203);江苏省"333高层次人才培养工程"项目(2007-4)

摘　　要：以冬虫夏草菌丝体为材料,经原生质体紫外诱变和再生进行优良菌种选育。实验结果表明:诱变30s的8号再生菌株为优良突变株,其生物量和虫草素产量分别比出发菌株提高55.13%和113.99%,结果表明原生质体诱变技术在冬虫夏草菌种选育中是可行的。Using protoplast of Cordyceps sinensis as the experimental material,screening high yield strains of biomass and cordycepin by mutagenesis of protoplast with UV and protoplast regeneration. The results showed that the No.8 strain of 30 s by mutated with UV was the best strain. The biomass and cordycepin yield were 55.13% and 113.99% higher than those of the original strain,respectively. The results showed else that it was the feasible of screening the best strain of Cordyceps sinensis by protoplast mutates with UV.

关 键 词：冬虫夏草 原生质体 诱变 虫草素 

分 类 号：S567.35[农业科学—中草药栽培] Q813.5[农业科学—作物学]