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机构地区:[1]中南林业科技大学材料科学与工程学院,长沙410004
出 处:《食品科技》2009年第12期222-225,共4页Food Science and Technology
基 金:国家科技部"十一五"科技支撑项目(2006BAD18B0404);湖南省科技厅科技攻关项目(05NK3056);中南林业科技大学校青年基金项目(07029B)
摘 要:以银杏叶为研究对象,设计正交实验分别优化了溶剂回流法、微波法、超声波法提取银杏黄酮的工艺条件并进行比较研究;运用扫描电镜,从微观角度探讨了微波、超声波在银杏叶活性成分提取过程中的强化传质机理。结果表明:在各自的优化提取工艺条件下,3种方法所得银杏黄酮得率分别为3.10%、4.09%和3.68%。通过对银杏叶细胞的微观结构变化比较分析可知,不同提取方法对植物细胞组织的影响程度明显不同;溶剂回流法不能破坏细胞,微波和超声波两种外场使细胞更易出现裂缝、破碎,降低传质阻力,微波提取法因产物得率较高、耗时较短具有明显优势。The technology conditions of solvent reflux extraction,microwave and ultrasonic extraction of flavonoids from Ginkgo biloba leaves were optimized and compared by orthogonal experiment. From microcosmic point scanning electron microscope was employed to study enhancing mass-transfer mechanism of the three methods.The results showed that under optimum extraction conditions,the flavonoids yield by these techniques were 3.10%,4.09%,3.68% respectively. The variation of cell microcosmic structure showed different extraction ways had different effect on cell organization. Microwave and ultrasonic fields made cell rupure and break more easier and lowered the mass-transfer resistance while solvent reflux extraction couldn't. For higher yield and less extraction time, microwave had obvious superiority.
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