温郁金脱毒组织培养技术研究  

Techniques of Virus-free Tissue Culture for Wenyujin Y.H Chen et C.Ling

作　　者：汪洪[1] 蔡小军[1] 于晓敏[1] 李校堃[1] 

机构地区：[1]温州医学院药学院,浙江温州325035

出　　处：《药物生物技术》2009年第6期565-567,共3页Pharmaceutical Biotechnology

基　　金：浙江省中医药管理局资助项目(2004C122);浙江省科技攻关重大项目(2005C13019);浙江省中医药重点研究项目计划(2006z019)

摘　　要：为了建立温郁金脱毒组培苗的快繁体系,以温郁金主根茎为材料,采用高温预处理催芽结合茎尖培养技术获得脱毒苗。结果表明:茎尖培养的最适培养基为MS+BA(6-苄基嘌呤)2.0 mg/L+NAA(萘乙酸)0.5 mg/L+蔗糖3%+琼脂5.0 g/L;不定芽增殖培养基为MS+6-BA 3.0 mg/L+蔗糖3%+琼脂5.0 mg/L;脱毒苗生根诱导最适培养基为MS+6-BA(6-苄基嘌呤)1.5 mg/L+NAA(萘乙酸)2.0 mg/L+蔗糖3%+琼脂5.0 g/L,用超薄电镜切片法和RT-PCR法检测病毒率,该茎尖再生苗脱毒率达78.6%。试管苗移栽后植株的成活率达90%以上,植物生长健壮,抗性增强,为温郁金种苗进一步工厂化快速繁殖提供了重要参考价值。To establish a virus-free tissue culture plantlets ofWerlyujin Y. H Chen et C. Ling, Rhizomes cultured at high temperature in order to induce buds and stem tips of the buds were cultured on MS media. Both electron microscope inspection and RT-PCR technique were used to confirm that plantlets growing from stem tips were escaped from virus. The obtained results indicated that the optimized medium for stem tip culture was MS＋6 - BA 2.0 mg/L＋NAA 0.5 mg/L＋sucrose 3%＋agar 5.0 g/L. The most suitable medium for producing rosette buds was MS＋6- BA 3.0 my/L＋ sucrose 3%＋ agar 5.0 g/L. The optimized medium for rooting was MS＋6 - BA 1.5 mg/L＋NAA 2.0 mg/L＋sucrose 3%＋agar 5.0 g/L. The virus-free rate was as high as 78.6% after 5 weeks. The survival rate of plantlets was over 90% after being transplanted for 30 days. The plantlets grew well and were improved in yield and quality. This research provided techniques for the application of Wenyujin Y. H Chen et C. Ling.

关 键 词：温郁金 脱毒 组织培养 

分 类 号：R284[医药卫生—中药学]