白桦苯丙氨酸解氨酶(PAL)基因的分离及其表达  

Expression and Isolation of Phenylalanine Ammonia-lyase (PAL) Gene in Betula platyphylla

作　　者：宋福南[1] 邢磊[1] 陈肃[1] 戴超[1] 刘雪梅[1] 

机构地区：[1]林木遗传育种与生物技术教育部重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2009年第12期14-17,47,共5页Journal of Northeast Forestry University

基　　金：教育部科学技术研究重点项目(109053);哈尔滨市科技创新人才研究专项资金项目(2008RFQXN009)

摘　　要：利用RT-PCR和RACE技术从白桦(Betulaplatyphylla)中克隆了编码苯丙氨酸解氨酶(PAL)的cD-NA,其2322bp的ORF编码773个氨基酸,其推导的氨基酸序列包含PAL-HAL和PAL2个功能域以及酶活性中心序列GTITASGDLVPLSYIA,该序列同其它5种植物的序列一致性为60%～73%,其中与美洲红(Rhizophoraman-gle)树最高为73.1%。以该序列构建了系统进化树,白桦与美洲红树聚为一类,其余3种裸子植物长白松、沙地海岸松和银杏聚为一类。BplPAL1基因在各组织中均有不同的转录表达,在次生木质部表达最强,其次是幼叶,在花序中的表达量较低,说明BplPAL1基因在各组织中的调控和表达是不同的。The eDNA （ BpIPAL1 ） encoding phenylalanine ammonia-lyase （PAL） were cloned from Betula platyphylla by reverse transcription polymerase chain reaction （RT-PCR） and 5＇ and 3＇ rapid amplification of cDNA ends （RACE）, which contains an open reading frame （ORF） （2 322 bp） encoding 773 amino acids. The amino acid sequences contain two functional domains of PAL-HAL and PAL, and the sequence of enzyme active site （GTITASGDLVPLSYIA）, which has 60% 73% of consistency with other five plant species and the consistency with Rhizophora mangle is the highest （73.1%）. Phylogenetic analysis of BplPAL1 with other plant species revealed that B. platyphylla and R. mangle formed a gymnosperm-type PAL subfamily, and Pinus sylvestris, Pinus pinaster, Ginkgo biloba formed a subfamily. Semi-quantitative RT- PCR analysis indicated that the expression of BplPAL1 genes was different in tissues, exhibiting the strongest in xylem, next in young leaves, and the weakest in inflorescences. The results suggest that BplPAL1 genes are differently regulated and expressed in tissues.

关 键 词：苯丙氨酸解氨酶 基因表达 木质素生物合成 抗病性 次生代谢 木材形成 

分 类 号：Q943.2[生物学—植物学]