大豆铁结合蛋白基因的克隆及其推定蛋白结构分析  

Cloning of the iron-binding protein gene from soybean and analysis of its putative protein structure

作　　者：陈新建[1] 王淑丽[1] 杨秋云[1] 陈军营[1] 

机构地区：[1]河南农业大学农学院,河南郑州450002

出　　处：《河南农业大学学报》2009年第6期587-590,共4页Journal of Henan Agricultural University

基　　金：国家转基因植物研究与产业化专项基金(JY03-B-19-2);河南省杰出人才创新基金(No.022100090)

摘　　要：根据GeneBank数据库中大豆类铁结合蛋白序列设计引物,通过反转录方法从豫豆8号中克隆到cDNA片段,测序结果表明,该基因cDNA全长752 bp,编码250氨基酸.利用DNAMAN软件进行序列比对分析发现,该cDNA的氨基酸序列与大豆(M64337.1)铁结合蛋白同源性高达98%以上.运用生物信息学软件及相关网站对其功能位点、结构功能域分析,结果显示,该基因具有1个FER-RITIN-LIKE功能域、2个铁结合蛋白基因家族铁结合区域的信号序列、6个磷酸化位点等重要功能位点.Primers were designed according to beans＇ ferritin sequence registered in GeneBank, Yu- dou 8 was used as experimental material and RT - PCR was employed in order to clone ferritin gene. A full-length cDNA, 752 bp, 250 amino acids encoded, was cloned. Sequence alignment revealed that there was above 98% identity between soybean （M64337.1） ferritin and current sequence. The putative protein structure features were analyzed by bioinformatics softwares and relevant web sites. Results showed that there are one FER-RITIN-LIKE function domain, two iron-bindi.ng sites, six phosphorylation sites, and other important sites in the protein.

关 键 词：铁结合蛋白基因 克隆 生物信息学 

分 类 号：S565.1[农业科学—作物学]