适用于AFLP分析的泡桐DNA提取方法研究  

Study on DNA extraction of AFLP reaction system for Paulownia plants

作　　者：张延召[1] 曹喜兵[1] 翟晓巧[1] 范国强[1] 

机构地区：[1]河南农业大学泡桐研究所,河南郑州450002

出　　处：《河南农业大学学报》2009年第6期610-614,共5页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(30571496);高等学校博士学科点专项科研基金项目(20050466003);中国博士后基金项目(20070410874)

摘　　要：分别采用3种方法提取了豫杂一号泡桐组培苗叶片DNA,并对其得率和质量进行了检测.结果表明,DNAkit,CTAB-Ⅰ和CTAB-Ⅱ法提取DNA的得率分别为0.250,0.284,0.312 mg.g-1;CTAB法提取的DNA片段大于DNAkit法;利用CTAB-Ⅱ法得到的DNA可被EcoRI,MspI和HpaⅡ完全酶切,该DNA经AFLP-PCR扩增电泳后,产物带型清晰,稳定,重复性好.CTAB-II法为泡桐DNA提取的最佳方法.The purity and yield of DNA extracted with 3 different kinds of methods from Paulownia tomentosa x Paulownia fortunei leaves were investigated with uhraviolet-spectrophotometer and molecular technique. The results indicated that the yield of DNA extracted with DNA kit method, CTAB- Ⅰ method and CTAB-Ⅱ method were O. 250,0. 284 and 0. 312 mg · g^-1 respectively, and the fragment of DNA extracted with CTAB methods was longer than that extracted with DNA kit. The DNA extracted with CTAB-Ⅱ could be completely digested with EcoRⅠ, Msp Ⅰ and HpaⅡ . And its band patterns of AFLP-PCR products were clear, stable and reproducible with gel electrophoresis. So CTAB-Ⅱ was considered to be the best DNA extraction method for Paulownia plants.

关 键 词：泡桐 DNA提取 限制性酶切 AFLP 

分 类 号：S792.43[农业科学—林木遗传育种]