粗提牛白细胞抗菌肽对小鼠免疫功能的影响  

Effect of antimicrobial bovine neutrophil extract on immune functions in mice

作　　者：金钺[1] 刘慧敏[1] 耿娟[1] 杨霞[1] 陈丽颖[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业大学学报》2009年第6期630-633,共4页Journal of Henan Agricultural University

基　　金：河南省科技攻关项目(0523010500);河南省重点科技攻关项目(072102130020)

摘　　要：将粗提牛白细胞抗菌肽分别以低、中、高剂量(1.5,7.5,15 g.L-1)灌胃小鼠,0.2 mL.只-1;对照组分别为灌施体积分数为0.01%醋酸溶液和10 g.L-1的牛血清白蛋白各0.2 mL.测定小鼠的细胞吞噬功能和免疫器官指数;同时采用流式细胞术测定小鼠外周血中T淋巴细胞亚群的总数及各亚群的百分数.结果表明,7.5 g.L-1的粗提牛白细胞抗菌肽能明显提高小鼠的免疫器官指数(P<0.05),增强小鼠的细胞吞噬功能(P<0.05)以及小鼠外周血CD3+,CD4+及CD8+T淋巴细胞的百分数(P<0.01),而高剂量组比中剂量有所降低.7.5 g.L-1的粗提牛白细胞抗菌肽能明显增强小鼠的免疫功能,提高机体的免疫水平.Bovine blood was collected and the antimicrobial components were extracted from leukocytes through ammonium chloride lyses, sonieation, centrifugation and freeze-drying. The extract was admin- istrated for two weeks by means of gavage in a daily dose of 0.2 mL at a protein concentration of 1.5, 7.5, 15 g ·L^-1 respectively, and the control groups were separately fed with 0.2 mL of 0.01% acetic acid and 10 g ·L^-1 BSA . The serum phagocytic rate, phagocytic index, and weight of the immune organs （spleen and thymus） were measured, whilst the total number and subgroups of T lymphoeytes were mounted through flow cytometry （FCM）. The results shows that at a dosage of 7.5 mg protein content, the extract improves the serum phagocytic index, which indicates the ability of phagoeytosis is enhanced （P 〈 0.05 ） ; the immune organ weight index increases significantly （P 〈 0.05 ） ; the percentages of CD3^＋ , CD4^＋ , and CD8^＋ T cells are also greatly improved （P 〈 0.01 ）. It may be concluded, therefore, that the bovine leukocyte extract can improve the level of both innate immunity and adaptive immune responses so that it can be promisingly applied in both human medicine and veterinary fields.

关 键 词：牛 抗菌肽 免疫 流式细胞术 T淋巴细胞 

分 类 号：S852[农业科学—基础兽医学]