药用植物灯盏花组织培养体系的建立  

Establishment of an Optimized Tissue Culture System for Erigeron breviscapus

作　　者：林丽飞[1] 陶发清[2] 刘春国 张灿邦[4] 胡先奇[5] 金秋[1] 白学贵[1] 熊力军[1] 

机构地区：[1]云南红河学院生物系,云南蒙自661100 [2]中国科学院昆明植物研究所,昆明650204 [3]云南省建水县临安镇农技推广中心,云南建水654300 [4]云南红河学院科技处,云南蒙自661100 [5]云南农业大学云南省植物病理重点实验室,昆明650201

出　　处：《西南大学学报（自然科学版）》2009年第12期82-86,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(60748002);云南省自然科学基金资助项目(2003C0044M);云南省科技计划资助项目(2006E0091M);红河学院硕士专项课题资助项目(XSZ05030)

摘　　要：以灯盏花(Erigeron breviscapus)叶片为外植体,比较不同激素及其浓度对愈伤组织的诱导与分化、继代增殖、生根的影响.结果表明:诱导愈伤组织最佳的培养基是MS+KT0.5mg/L+2,4-D10mg/L和MS+2,4-D0.5mg/L+6-BA0.5mg/L,诱导率为100%.二者相比,前者长出的愈伤组织较为紧密,而后者松散性好.MS+6-BA0.5mg/L+10%香蕉汁是诱导芽最佳的培养基,达87%;加入0.3mg/LNAA的1/2MS培养基对生根较为有利,达83%.With the leaves of E.breviscapus as the explants,the effects of different combinations of auxin and cytokinin at different concentrations on callus induction,subculture and rooting were investigated.The result showed that both the media MS＋KT 0.5 mg/L＋2,4-D 10 mg/L and MS＋2,4-D 0.5 mg/L＋6-BA 0.5 mg/L were suitable for callus induction,the induction rate being 100%.The medium MS＋6-BA 0.5 mg/L＋10% banana juice was the best for plantlet differentiation,with an inductivity of 87%.1/2MS＋0.3 mg/L NAA was advantageous to rooting of the shoots and the root formation rate was 83%.

关 键 词：灯盏花 组织培养 愈伤组织 芽和根诱导 

分 类 号：Q949.783.5[生物学—植物学]