促血管生成素-1、2及其受体在寻常性银屑病中的表达  

Expression of angiopoietin-1, -2 and their receptor, tyrosine kinase with immunoglobulin and epidermal growth factor homology domain-2 （Tie-2） in patients with psoriasis vulgaris

作　　者：罗权 张锡宝 周欣 林玲 李雪梅 朱慧兰 刘玉梅 何玉清 曾抗[2] 

机构地区：[1]广州市皮肤病防治研究所,510095 [2]南方医科大学南方医院皮肤科

出　　处：《中华皮肤科杂志》2009年第12期821-823,共3页Chinese Journal of Dermatology

基　　金：广东省自然科学基金（07001961）;广州市医药卫生科研重点项目（2006-ZDi-07）

摘　　要：目的探讨促血管生成素-1（Ang—1）、促血管生成素-2（Ang-2）及其受体Tie-2在寻常性银屑病皮损组织中的意义。方法免疫组化SABC法检测30例寻常性银屑病患者皮损中的Ang—1、Ang-2及其受体Tie-2蛋白表达，以CD34标记新生血管内皮并计数微血管密度（MVD）。另取15例正常人皮肤组织作为对照组。结果寻常性银屑病患者皮损处Ang-2及其受体Tie-2表达明显强于正常人对照组（P〈0．01），而Ang-1蛋白的表达与正常人对照组表达差异无统计学意义。进行期与静止期比较，Ang-2及Tie-2表达差异均有统计学意义（P〈0．05），而Ang-1蛋白的表达差异无统计学意义（P〉0．05）。寻常性银屑病患者皮损中MVD值明显高于正常人对照组（P〈0．01），高密度组（MVD≥15．46）与低密度组（MVD〈15．46）的Ang-1表达差异无统计学意义（P〉0．05），但Ang-2及其受体Tie-2表达差异有统计学意义（P〈0．05）。相关性分析表明，银屑病皮损Ang-1与Ang-2蛋白表达水平呈负相关（r=-0．521，P〈0．05）；MVD值与Ang-2蛋白表达呈正相关关系（r=0．656，P〈0．01），与Ang-1蛋白表达无相关性（r=0．237，P〉0．05）。结论Ang-2及其受体Tie-2与银屑病的血管生成关系密切，促血管生成素及其受体系统在银屑病新生血管生成中的调节作用可能是以Ang-2的作用为主导的。Objective To investigate the expressions ofangiopoietin （Ang）-1, -2 and their receptor, Tie-2 in patients with psoriasis vulgaris and their significance. Methods Tissue samples were collected from 30 patients with psoriasis vulgaris and 15 normal controls. Streptavidin-biotin complex （SABC） method was used to detect the expression of Ang-1, -2 and Tie-2 in these samples. Microvessel density （MVD） was assessed by CD34 staining. Results The expressions of Ang-2 and Tie-2 were significantly higher in patients with psoriasis vulgaris than in the normal controls （7.65 ±2.14 vs 2.54 ± 0.91,6.09 ± 1.97 vs 1.95 ± 0.62, both P 〈 0.01 ）, and higher in patients with active psoriasis than in those with inactive psoriasis （9.85 ± 2.42 vs 4.86 ± 1.63, 8.43 ± 1.98 vs 4.57 ±1.92, both P〈 0.05）, whereas no significant difference was observed in the expression level ofAng-1 between patients and controls or between patients with active psoriasis and those with inactive psoriasis （all P 〉 0.05 ）. Increased MVD was observed in patients with psoriasis vulgaris compared with the controls （ 15.46 ± 5.28 vs 2.68 ± 0.79, P 〈 0.01 ）. Also, the expression levels of Ang-2 and Tie-2 were significantly different between patients with high MVD and those with low MVD （both P 〈 0.05 ）, while no difference was noted in the expression of Ang-1 （P 〉 0.05 ）. In psoriatic lesions, a negative correlation was observed between the expression of Ang-1 and Ang-2 （r = -0.521, P 〈 0.05 ）, and MVD was negatively correlated with the expression of Ang-2 （r = 0.656, P 〈 0.01 ） but unrelated to the expression of Ang-1 （r =0.237, P 〉 0.05 ）. Conclusions It is demonstrated that Ang-2 and Tie-2 closely correlate with the angiogenesis in psoriasis, and Ang-2 plays a dominant role in the regulation ofangiogenesis in psoriasis.

关 键 词：银屑病 血管生成素类 微血管密度 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]