光栅空间滤波方法实现显微光切片  

Method of Obtaining Optical Sectioning by Grating Spatial Filter

作　　者：李晶 赵海燕[2] 

机构地区：[1]昆明总医院医学工程科,昆明650032 [2]云南省第一人民医院眼科,昆明650032

出　　处：《生物医学工程学杂志》2009年第6期1218-1221,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30770540);成都军区医学科研计划课题资助(MB07026)

摘　　要：探索应用数字空间滤波方法,在普通显微镜下,构建结构光,实现宽场光学切片技术。对技术方法进行了理论推导,在去除光栅条纹方面,通过修改图像的傅立叶频谱,来消除光栅条纹,从而使线形去光栅方法得以改进。分析讨论了本技术硬件软件实现及其优劣。Based on the theory of digital spatial filter, we have developed a new method for obtaining optical sectioning by using a grating structured light in a conventional microscope. Theoretical derivation of technology was presented in the paper. In order to remove the unwanted grid pattern superimposed, we have changed the FFTs spectrum of image and have found a method better than the original linear method. Lastly we have explained how to realize the method as well as its advantage and disadvantage.

关 键 词：数字图像处理 傅立叶变换 光栅 空间滤波 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术]