动脉粥样硬化进程中单核细胞-巨噬细胞的黏弹性比较实验  

Comparative Study on the Viscoelastic Properties of Phagocytes of Atherosclerotic Origin

作　　者：曾伟[1,2] 邓小燕[1] 秦建[3] 

机构地区：[1]北京航空航天大学生物与医学工程学院,北京100191 [2]四川理工学院电子与信息工程系,自贡643000 [3]重庆大学生物工程学院,重庆400044

出　　处：《生物医学工程学杂志》2009年第6期1264-1266,1280,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10572017;10632010;30670517)

摘　　要：运用微管吸吮技术定量测量了动脉粥样硬化过程中吞噬细胞的黏弹性特性。选择标准线性固体模型(Kelvin模型)拟合实验结果,并用Kelvin模型的三个黏弹性参数来比较动脉粥样硬化过程中不同阶段吞噬细胞的力学特性。实验结果表明,在动脉粥样硬化形成以前,单核细胞在进入血管内膜变成巨噬细胞后,其细胞的变形能力就已经开始下降,细胞的刚性增加;在动脉粥样硬化初步形成后,泡沫细胞的变形能力进一步下降。我们认为,吞噬细胞在吞噬低密度脂蛋白后变形能力的恶化可能会直接影响细胞的爬行能力,致使泡沫细胞滞留于动脉血管内膜,导致动脉粥样硬化斑块的形成。这一研究结果对于动脉粥样硬化的研究可能具有重要的参考意义。Abstract A micropipette aspiration technique was adopted in this study on the viscoelastic properties of phagocytes of atherosclerotic origin. A standard linear solid model （Kelvin model） was employed to fit the experimental data, and the 3 viscoelastic coefficients of the model were used to compare the mechanical properties of the phagocytes in different phases during atherosclerosis development. The experimental results indicated that prior to the formation of atherosclerosis, the deformability of the marcopahges matured from monocytes decreased, and their rigidity increased. At the initial stage of atherosclerosis formation, the deformability of the foam-cells decreased further. We believe that the deterioration in the deformability of the cells might reduce their mobility within the arterial wall, thus leading to the genesis of atherosclerosis caused by the stagnation and accumulation of the cells laden with atherogenie lipids within the arterial wall. This finding may have important implication in the researches on arteriosclerosis.

关 键 词：动脉粥样硬化 吞噬细胞 黏弹性 微管吸吮技术 

分 类 号：R543[医药卫生—心血管疾病]