构建椎间盘组织工程的人骨髓间充质干细胞的分离及三维培养  

The Three-dimensional Culture of Adult Mesenchymal Stem Cells for Intervertebral Disc Tissue Engineering

作　　者：丰干钧[1] 刘浩[1] 邓力[2] 陈晓禾[2] 李秀琼[2] 赵献峰[1] 梁涛[1] 

机构地区：[1]四川大学华西医院骨科,成都610041 [2]四川大学华西医院干细胞与组织工程研究室,成都610041

出　　处：《生物医学工程学杂志》2009年第6期1300-1305,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30572163)

摘　　要：椎间盘退变(Degenerative disc disease,DDD)是下腰痛的主要原因。当前临床治疗主要目的在于恢复生物力学功能和缓解症状。而椎间盘组织工程的兴起为DDD的治疗提供了新方法,其中干细胞是用于椎间盘组织工程的重要种子细胞。在本实验中,我们研究人骨髓间充质干细胞(MSCs)体外分离培养的细胞学特性以及在Pellet三维培养体系下构建椎间盘组织工程细胞的可行性。结果提示该方法培养的MSCs具较高的纯度,且体外培养具有较强的增殖能力,短时间内可大量扩增,达到组织工程需要的种子细胞数量要求,且细胞保持未分化状态,具有良好的生物安全性。并能表达Ⅱ型胶原和蛋白多糖等椎间盘细胞外基质,因此是椎间盘组织工程理想的种子细胞。Intervertebral disc （IVD） degeneration is one of the major causes of low back pain. As current clinical treatments are aimed at restoring biomechanical function and providing symptomatic relief, the methods focused on biological repair have aroused interest and several tissue engineering approaches using different cell types have been proposed. Owing to the unsuitable nature of degenerate cells for tissue engineering, attention has been given to the use of mesenchymal stem ceils （MSCs）. In this connection, we have made a study on the characteristics of MSCs derived from adult bone marrow and on the feasibility of constructing IVD tissue-engineering cell under a Three-Dimensional Pellet Culture System. The human bone marrow MSCs were isolated and purified with density gradient solution and attachment-independent culture system. MSCs isolated using this method are a homogeneous population as indicated by morphology and other criteria. They have the capacity for self-renewal and proliferation, and the multilineage potential to differentiate.

关 键 词：间充质干细胞 分化软骨形成椎间盘组织工程 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]