体外展示技术  

In Vitro Display Technologies

作　　者：阎松[1,2] 张翼[1] 吕红丽[1] 董学伟[1] 唐超[3] 穆军[1] 

机构地区：[1]大连交通大学环境与化学工程学院,大连116028 [2]中国科学院海洋研究所实验海洋生物学重点实验,青岛266071 [3]青岛农业大学生命科学学院,青岛266109

出　　处：《生物医学工程学杂志》2009年第6期1367-1371,共5页Journal of Biomedical Engineering

基　　金：国家高技术研究发展计划(863计划)项目资助(2006AA09Z426);国家自然科学基金资助项目(30472043)

摘　　要：以体外选择方法筛选不同功能的核酸、肽和蛋白是近年的研究热点。体外展示方法不受细胞转化效率限制,可建立多达1013～1014分子的大容量库。应用最广泛的体外展示技术是核糖体展示和mRNA展示技术。核糖体展示通过在体外翻译反应时终止蛋白质合成,获得mRNA-核糖体-新生肽复合物。mRNA展示利用小的连接分子,主要是嘌呤霉素形成mRNA-蛋白质复合物,这些mRNA-蛋白质复合物纯化之后用于体外筛选。体外展示技术为体外筛选和蛋白质进化提供了另外的途径,本文将对核糖体展示和mRNA展示技术的原理、方法和应用研究作一综述。The application of in vitro selection method to isolate nucleic acids, peptides and proteins according to their functions has been studied intensively in recent years. In vitro display technologies are not limited by cellular transformation efficiencies; thus, very large libraries of up to 10^13-10^14 members can be built. The most popular in vitro display technologies are ribosome display and mRNA display; ribosome display achieves the mRNA-ribosomenascent peptide complexes by stalling the translating ribosome in an in vitro translation reaction. In mRNA display, the mRNA-protein complex is achieved by binding the two macromolecules through a small adaptor molecule, typically puromycin; these mRNA-peptide fusions can then be purified and subjected to in vitro selection. In vitro display technologies provide a different approach to the in vitro selection and directed evolution of peptides and proteins. This review focuses on the principle and method of ribosome display and mRNA display technologies, and discusses their applications.

关 键 词：体外展示技术 核糖体展示 mRNA展示 

分 类 号：Q78[生物学—分子生物学]