抗菌肽基因异源表达的研究进展  

Heterogenous Expression of Antimicrobial Peptides

作　　者：宋珊珊[1,2] 胡国斌[1] 董先智[2] 

机构地区：[1]中国海洋大学海洋生命学院,青岛266003 [2]中国科学院生物物理研究所,北京100101

出　　处：《生物医学工程学杂志》2009年第6期1372-1375,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30671604)

摘　　要：抗菌肽是生物界中广泛存在的一类具有抗菌活性的短肽,是天然免疫系统中的重要防御成分。抗菌肽具有抗菌谱广、活性稳定,不易产生耐药性等优点,因而在食品、饲料、医药领域具有重要的应用价值。目前抗菌肽规模化生产的主要困难在于如何降低成本、大量获得活力稳定的多肽产品。由于化学合成的成本较高,利用基因工程技术构建高效的生物表达系统成为实现这一目标的关键途径。本文对抗菌肽在大肠杆菌、酵母、昆虫、动植物细胞等表达系统中异源表达的研究进展进行了综述。Antimicrobial peptides （AMPs）, a class of short proteins with a broad spectrum of antibacterial activities, are isolated from a wide variety of animals, both vertebrates and invertebrates, and plants as well as from bacteria and fungi. They are a key component of the innate immune response in most multicellular organisms. Owing to their potent, broad-spectrum antibacterial activities and uneasy developing of drug resistance, these peptides are of great clinical significance. However, preparation of AMPs at a large scale is a severe challenge to the development of the commercial products. Undoubtedly, construction of high-level biological expression systems for the production of AMPs is the key in its clinical application process. Herein, we summarize the progress in researches on heterogenous expression of AMPs in prokaryotic expression systems and eukaryotie expression systems.

关 键 词：抗菌肽 原核表达 真核表达 

分 类 号：Q78[生物学—分子生物学]