溃疡性结肠炎患者外周血单个核细胞中TREM-1mRNA的表达水平  

The mRNA expression of triggering receptor expressed on myeloid cells-1 in peripheral blood monocytes of patients with ulcerative colitis

作　　者：张蜀澜[1] 李丽君[1] 李永哲[1] 沈冰冰[2] 胡朝军[1] 朱峰[2] 钱家鸣[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院风湿免疫科,北京100032 [2]中国医学科学院北京协和医学院北京协和医院消化科,北京100032

出　　处：《中华检验医学杂志》2009年第12期1349-1353,共5页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目（30471617,30640084,30872331）.

摘　　要：目的通过比较髓系细胞触发受体-1（TREM-1）mRNA在活动期和缓解期溃疡性结肠炎（UC）患者以及健康体检者外周血单个核细胞（PBMC）中的表达水平，探讨TREM-1与UC疾病活动性的关系。方法依据结肠镜检和病理活检结果，将UC患者分为活动期和缓解期2组，采用实时荧光定量逆转录（FQ-RT）-PCR检测70例UC患者（活动期患者38例，缓解期患者32例）和20名健康体检者PBMC中TREM-1 mRNA的表达水平，采用相对定量△Ct值比较基因表达水平的高低，并比较TREM-1 mRNA表达水平与ESR和C反应蛋白（CRP）的相关性。结果UC活动期患者PBMC中TREM-1 mRNA的表达水平（4.19±1.86）显著高于UC缓解期患者（5.29±1.71，P=0.007）和健康体检者（5.19±1.04，P=0.032），而UC缓解期患者与健康对照组差异无统计学意义（P=0.892）；TREM-1 mRNA表达的受试者操作特性曲线（ROC）下面积为0.763（P〈0.001），表明TREM-1 mRNA表达水平对UC疾病活动性具有诊断价值；当△Ct值为4.63时，是判断UC活动性的最佳临界点，其诊断的敏感度和特异度分别为73.7％和68.7％；UC活动期患者中TREM-1 mRNA的表达与ESR显著相关（r=0.582，P=0.03），而与血清中CRP水平不相关（r=0.447，P=0.055）。结论TREM-1 mRNA的表达与UC的活动程度明显相关，TREM-1可能参与了UC的炎症进程。TREM-1 mRNA的表达水平可能与ESR相关，而与血清中CRP水平不相关。Objective To compare the levels of mRNA expression of triggering receptor expressed in myeloid cells-1（TREM-1） in peripheral blood mononuclear cell （PBMC） of active ulcerative colitis （UC） patients, remittent UC patients and healthy controls and find out the relationship between TREM-1 and disease activity. Methods The patients with UC were divided into active group and remittent group according to results from colonoscopy and biopsy. The expressions of TREM-1 mRNA in PBMC were detected by real-time quantitative polymerase chain reaction （FQ-RT-PCR） in 70 UC patients （38 active patients, 32 remittent patients） and 20 healthy controls, and the levels of gene expression were analyzed by the relative quantitative △Ct values. The correlations between the expressions of TREM-1 mRNA, ESR and CRP were identified by analyzing the relationship between the level of TREM-1 mRNA expression and the quantity of ESR and CRP in active UC patients. Results Expressions of TREM-1 mRNA in active UC patients（4.19±1.86） were higher than those of remittent UC patients （5.29±1.71, P = 0.007）and healthy controls （5.19±1.04,P = 0.032）. There was no statistical difference between remittent UC patients and healthy controls （ P = 0. 892）. The area under receiver operating characteristic （ ROC ） curve （0.763） was above O. 5 （ P 〈 0.001） ,showing that TREM-1 mRNA was of value for prediction the disease activity in patients with UC.△Ct of 4.63 was the best threshold to determine the disease activity and its diagnostic sensitivity and specificity were 73.7% and 68.7%, respectively. In active UC patients, expression of TREM-1 mRNA was correlated with ESR（r=0.582, P = 0.03）, but there was no correlation with CRP（ r = 0.447, P = 0.055）. Conclusions Expression of TREM-1 mRNA is significantly elevates in active UC patients, which suggests that TREM-1 might be involved in the pathogenesis of UC. In active UC patients, there is a correlation between expression of TREM-1 mRNA and ESR, but

关 键 词：结肠炎 溃疡性 白细胞 单核 膜糖蛋白类 受体 免疫 逆转录聚合酶链反应 

分 类 号：R686[医药卫生—骨科学]