冰片对三七皂苷R1和人参皂苷Rg1,Re家兔组织分布的影响  

Effect of borneol on the tissue distribution of notoginseng R1,ginsenoside Rg1 and Re in rabbits

作　　者：王世祥[1] 苗文莉[2] 房敏峰[1] 南叶飞[1] 孟雪[1] 于洁[1] 郑晓晖[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西安交通大学中草药现代化研究及工程中心,陕西西安710061

出　　处：《第四军医大学学报》2009年第23期2750-2752,共3页Journal of the Fourth Military Medical University

基　　金：教育部重点项目(207151);陕西省教育厅自然科学项目(07JK399)

摘　　要：目的:建立三七皂苷R1和人参皂苷Rg1,Re家兔组织中的固相萃取-高效液相色谱(SPE-HPLC)分析方法,并研究冰片对三七中三七皂苷R1,人参皂苷Rg1,Re在家兔体内组织分布的影响.方法:空白组、三七组、配伍组家兔于服药后30 min处死,取心、肝、大脑、肺、肾组织,匀浆后取上清液2.0 mL,固相萃取法去除干扰物质,收集70%甲醇洗脱液,HPLC测定三种指标成分在各组织中的含量.结果:三种皂苷在各组织中的含量均有增加,三七皂苷R1的变化最为显著.结论:冰片可显著提高三七皂苷R1、人参皂苷Rg1,Re的在脏器组织中的分布,从而提高三七的疗效.AIM：To establish a method for determination of notoginseng R1,ginsenoside Rg1 and Re in rabbit tissue by solid-phase extraction-high performance liquid chromatography（SPE-HPLC）,and investigate the effect of borneol on the tissue distribution of notoginsenoside R1,ginsenoside Rg1 and Re in Panax notoginseng in rabbits.METHODS：Rabbits were divided into 3 groups of control,Panax notoginseng and compatable randomly,and were sacrificed at 30.0 min to collect the tissues of heart,liver,brain,spleen and kidney after administration.The tissuesamples were homogenated and the suspension were collected for further use.SPE method was used to remove the interference substance prior to the collection of 70% methanol elution.HPLC method was employed for the determination of the above three saponins.RESULTS：The contents of the three saponins were all increased obviously in the tissues,and the increasing intend of notoginsenoside R1 is the most obvious.CONCLUSION：Borenol could increase the distributions of notoginsenoside R1,ginsenoside Rg1 and Re to tissue,and enhanced the therapeutic effect of Panax notoginseng.

关 键 词：三七 冰片 高效液相色谱 组织分布 

分 类 号：R917[医药卫生—药物分析学]