当归多糖对小鼠骨髓和外周血单个核细胞黏附分子表达及黏附功能的影响  

Effects of angelica polysaccharides on expression of adhesion molecules and adhesive function of murine bone marrow and peripheral blood mononuclear cells

作　　者：张雁[1] 杜小丽[1] 吴宏[1] 黎晓莉[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室组织胚胎学教研室,重庆400016

出　　处：《第四军医大学学报》2009年第23期2753-2756,共4页Journal of the Fourth Military Medical University

基　　金：重庆市自然科学基金计划项目(2007BB5284)

摘　　要：目的:研究当归多糖(APS)对小鼠骨髓和外周血单个核细胞(MNC)黏附分子表达及黏附功能的影响,旨在为APS在造血调控和动员方面的机制提供实验依据.方法:流式细胞术检测APS对小鼠骨髓和外周血MNC CD49d和CD44的表达影响;MTT法检测APS体内外作用对小鼠骨髓和外周血MNC黏附功能的影响.结果:APS(4 mg/kg)体内作用后骨髓和外周血MNC表面CD49d表达的阳性率均明显下降(P<0.01);骨髓MNC表面CD44也有所下降(P<0.05),外周血MNC表面CD44无明显变化(P>0.05).APS(4 mg/kg)体内作用后,小鼠骨髓和外周血MNC对纤维连接蛋白(FN)的黏附率明显低于NS组(P<0.01),且APS组与rhG-CSF组比较无显著性差异(P>0.05);除50 mg/L APS组以外各浓度实验组均能使小鼠骨髓和外周血MNC对FN的黏附率减弱(P<0.05),同一APS浓度体外作用后各作用时间点的骨髓和外周血MNC黏附率均无明显差异(P>0.05).结论:APS能降低骨髓的CD49d和CD44及外周血的CD49d的表达,且体内外作用均能降低骨髓和外周血MNC对FN的黏附率.AIM：To explore the effects of angelica polysaccharides（APS） on the expression of adhesion molecule and adhesive function in murine bone marrow and peripheral blood mononuclear cells（MNC）,so as to provide experimental evidence of APS on hematopoietic regulation and the mechanism of mobilizing.METHODS：The effects of APS on expression of CD49d and CD44 in murine bone marrow and peripheral blood MNC were analyzed by flow cytometry;the effects of APS on adhesive function in vitro and in vivo were evaluated by MTT assay.RESULTS：In APS group（4 mg/kg）：the percent of CD49d＇ expression on murine bone marrow and peripheral blood MNC obviously decreased（P〈0.01）,and the percent of CD44＇ expression on bone marrow MNC also decreased（P〈0.05）,but we did not observe any change of CD44＇ expression on bone marrow MNC（P〉0.05）;In APS group（4 mg/kg）：adhesive function of bone marrow and peripheral blood MNC to fibronectin（FN） was inferior to control group（P〈0.01）,but APS group did not have significant change with rhG-CSF group（P〉0.05）;With APS in vitro,the adhesive function of bone marrow and peripheral blood MNC to FN decreased in all the groups（without 50 μg/mL APS group）,APS groups of the same concentration did not have any change at different times（P〉0.05）.CONCLUSION：APS can decrease the expression of CD49d and CD44 in murine bone marrow and peripheral blood MNC,adhesive function in vitro and in vivo also decreased.

关 键 词：当归多糖 粘附分子 CD49D CD44 黏附功能 FN 

分 类 号：R285.5[医药卫生—中药学]