804G细胞外间质对体外培养牛角膜内皮细胞增殖的影响  

The effect of extracellular matrix of 804G cells on proliferation of bovine corneal endothelial cells in vitro

作　　者：汪渊[1] 桂淑玉[1] 

机构地区：[1]安徽医科大学生物化学教研室和分子生物学实验室

出　　处：《中国药理学通报》1998年第4期348-350,共3页Chinese Pharmacological Bulletin

摘　　要：目的研究细胞外间质对牛角膜内皮细胞增殖的影响。方法以８０４Ｇ细胞外间质作为牛角膜内皮细胞培养的基础，用免疫荧光分析结合素α６的表达，并测定酸性磷酸酶活性分析牛角膜内皮细胞增殖。结果生长于８０４Ｇ细胞外间质上的牛角膜内皮细胞结合素α６表达增加，酸性磷酸酶活性明显高于对照组。AIM To study the effect of extracellular matrix on bovine corneal endothelial cells. METHODS The integrin α6 expression of bovine corneal endothelial cells cultured on 804G extracellular matrix was observed by immuno fluorescence and acidic phophatase was measured. RESULTS The integrin α6 of bovine corneal endothelial cells cultured on 804G extracellular matrix was overexpression and activity of acidic phosphatase was significantly higher than control. CONCLUSION 804G extracellular matrix can lead to proliferation of bovine corneal endothelial cells in vitro.

关 键 词：细胞外间质 角膜 内皮细胞 细胞增殖 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]