体外受精-胚胎移植中新鲜睾丸精子与解冻后睾丸精子助孕结局比较  

In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa

作　　者：陈巧莉[1] 刘军霞[1] 裴莉[1] 韩伟[1] 曾品鸿[1] 叶虹[1] 黄国宁[1] 

机构地区：[1]重庆市妇幼保健院,400013

出　　处：《重庆医学》2009年第24期3063-3064,3069,共3页Chongqing medicine

摘　　要：目的比较单精子卵泡浆内显微注射受精助孕(intracytoplasmic sperminjection,ICSI)中,使用新鲜睾丸精子与解冻后睾丸精子两组的助孕结局。方法回顾性分析2002年1月至2008年8月在本所接受ICSI助孕的272个周期,其中117个周期使用新鲜睾丸精子,为A组;155个周期使用解冻后睾丸精子,为B组。结果(1)A、B两组受精率为77.1%vs 78.9%、优质胚胎率:20.7%vs 17.5%,两组比较差异无统计学意义。(2)妊娠及出生结局分析,A、B组临床妊娠率、着床率、流产率分别为48.7%vs 52.3%、26.9%vs 32.3%、8.8%vs 14.8%。足月活产率,低体重儿发生率分别为63.2%vs 55.6%、20.3%vs29.5%,两组比较差异均无统计学意义。结论体外受精-胚胎移植中采用解冻睾丸精子其助孕结局与新鲜睾丸精子相同,解冻后睾丸精子的使用可以最大限度减少反复睾丸活检对睾丸组织的损伤,增加睾丸组织的利用率。Objective To compare the outcomes of inlracytoplasmic sperm injection （ICSI） with fresh and cryopreserved testicular spermatozoa in the patients with obstructive and nonobstructive azoospermia. Methods A retrospective analysis was performed on 264 patients involving 272 ICSI cycles undergoing testicular sperm extraction during January 2002 to August 2008; the cycles were classified by ICSI with fresh or frozen-thawed spermatozoa. The fertilization rates,embryo quality,embryo implantation rates, clinical pregnancy rates per cycle,and delivery and spontaneous abortion rates were compared between the two groups. Results No statistically significant differences were found in each parameter examined between the groups. Fertilization rates were 77.1% with fresh vs 78.9% with frozen thawed testicular sperm; the good quality embryo rate were 20.7% vs 17.5%,implantation rates 26. 9% vs 32.3% ,pregnancy rates per cycle：48.7% vs 52.3% ,and abortion rates was 8.8% in frozen thawed testicular sperm,no sta tistically significant differences with the fresh group （P=0. 288）. Conclusion No differences were found in ICSI outcomes between cryopreserved and fresh testicular sperm. In addltion,cryopreservation provides several advantages for the patients and reproductive team.

关 键 词：单精子卵泡浆内显微注射受精助孕 经皮睾丸活检术 睾丸精子 妊娠率 

分 类 号：R714.8[医药卫生—妇产科学]