n-3多不饱和脂肪酸对高脂饲养大鼠大血管的保护作用机制  

Protective effects of n-3 polyunsaturated fatty acids on macrovascular disease induced by high fat-fed in rats

作　　者：肖彧君[1] 母义明[1] 潘长玉[1] 陆菊明[1] 柳红芳[1] 詹晓蓉[1] 陆祖谦[1] 

机构地区：[1]解放军总医院内分泌科,北京100853

出　　处：《军医进修学院学报》2009年第6期898-900,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的探讨n-3脂肪酸对高脂诱发的胰岛素抵抗大鼠的血管内皮细胞的保护机制。方法45只Wistar雄性大鼠随机分为对照组、高脂饲养（HF）组和HF＋n-3脂肪酸干预组。饲养11周后处死大鼠,测定血糖、胰岛素、血脂、体重以及内脏脂肪含量、主动脉组织形态学改变等,免疫组织化学法测定主动脉血管内皮细胞的ICAM-1、MCP-1和NF-κB/IκBα蛋白表达。结果n-3脂肪酸组大鼠体重第1-11周均明显降低,空腹血糖、血清胰岛素、TG、TC、LDL-C、总FFA和内脏脂肪占体重百分比均明显低于HF组,HDL-C则明显高于HF组,与对照组无明显差别;n-3脂肪酸组大鼠主动脉主动脉内膜完整无内皮细胞脱落现象,内膜下泡沫细胞及炎细胞浸润减少,内膜的NF-κB呈弱阳性表达,IκBα呈阳性表达,ICAM-1和MCP-1均呈阴性或弱阳性表达。结论n-3脂肪酸能有效阻断高脂饲养大鼠的胰岛素抵抗和大血管病变。Objective To investigate the protective effects of n-3 polyunsaturated fatty acids （PUFA） on endothelial cell damage induced by high fat in insulin-resistant rats. Methods Forty-five Wistar male rats aged 5-6 weeks were randomly divided into control group, high fat-fed （HF） group and n-3 PUFA group. The rats were sacrificed after 11 weeks. Levels of blood glucose, insulin, lipid, visceral fat and expression of ICAM-1, MCP-1 and NF- κ B/I κ B were measured. Body weight and histological change in aorta were detected. Results The body weight, visceral fat and body weight ration, as well as the levels of blood glucose, insulin, triglyceride, total cholesterol, LDL, and total FFA, were significantly lower in high fat-fed group than in control group （P〈0.01）. The aortic tunica intima was intact, the number of foam cells was decreased, and inflammatory ceils infiltrated into the med-intima smooth muscle of the n-3 PUFA group. However, with no such changes were observed in control and n-3 PUFAs groups. Little or no expression of NF- κ B, ICAM-1 and MCP-1, and a high expression level of I κ Bα were detected in aortic tunica intima of the n-3 PUFA and control groups, while high expression levels of NF- κ B, ICAM-1 and MCP-1, and little expression of I κ Bα were detected in aortic tunica intima of the high fat-fed group. Conclusion n-3 PUFA can effectively prevent insulin resistance and macrovascular diseases.

关 键 词：脂肪酸类 ω3 糖尿病血管病变 大鼠 

分 类 号：R58[医药卫生—内分泌]