饮食限制对运动训练大鼠脑干5-HT、5-HIAA及色氨酸羟化酶的影响  

Effects of Diet Restriction on the Contents of 5-HT,5-HIAA and Its Key Enzyme TPH in Rats Brain Stem with Exercise

作　　者：史仍飞[1] 张磊[2] 袁海平[1] 张平[1] 

机构地区：[1]上海体育学院运动科学学院,上海200438 [2]江西蓝天学院,江西南昌330098

出　　处：《体育科学》2009年第12期60-63,92,共5页China Sport Science

基　　金：上海市教育委员会科研基金(06IZ003);国家自然科学基金青年项目(30800542);上海市第2期重点学科建设项目(S30802)

摘　　要：目的:通过检测不同比例饮食限制对运动大鼠脑组织5-HT含量及其关键酶色氨酸羟化酶的影响,探讨饮食限制与运动性中枢疲劳的关系;方法:以40只成年雄性SD大鼠为实验对象,建立不同限制饮食动物模型。实验分组为安静对照组(NC)、100%饮食运动组(100%DR)、70%饮食运动组(70%DR)、50%饮食运动组(50%DR)、30%饮食运动组(30%DR),每组8只,饮食限制时间为10天,运动方案为每天进行游泳训练30min。采用高效液相色谱法测定脑干区5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)含量;采用RT-PCR分析脑干色氨酸羟化酶TPHmRNA表达量的变化;结果:1)各运动组大鼠脑干5-HT含量均高于安静对照组,但仅有30%饮食运动组有显著性增加(P<0.05);2)5-羟吲哚乙酸含量表现为30%DR组显著高于对照组和100%DR组(P<0.05);3)大鼠脑干区TPHmRNA的表达水平显示,30%DR组显著高于NC组和100%DR运动组(P<0.05),与100%DR组相比,各限食组均有显著性差异(P<0.05);结论:不同比例的饮食限制导致大鼠脑干5-HT和5-HIAA的生成增加,且增加量与饮食限制程度有关,其原因可能与饮食限制促进脑干TPH-mRNA的表达有关。Objective：To investigate the relationship between the central fatigue and dietary restriction and examine the contents of 5-HT and 5-HIAA and its key enzyme TPH in rat brain stem respond to dietary restriction. Methods：40 male Sprague-Dawley rats were used to establish different levels of dietary restricted models （normal control group, 100% dietary restriction group, 70 % dietary restriction group, 50 % dietary restriction group, 30 % dietary restricted group, dietary restriction duration of 10 days）. Concentrations of 5-hydroxy-tryptamine （5- HT）, 5-hydrox-yindolacetic acid （5-HIAA） in brain stem were measured using High Performance Liquid Chromatography （HPLC）, and the expression of TPH mRNA in brain stem was determined by semi-quantitative reverse-transcription polymerase chain reaction （RT-PCR）. Results.. 1. Compared with the normal control group, the concentrations of 5-HT in the brain stem increased significantly in 30 %dietary restricted group （P〈0.05）. 2. Compared with the normal control group and 100 % dietary restricted group, the concentrations of 5-HIAA in the brain stem increased significantly in 30%dietary restricted group （P〈0.05）. 3. The expressions of TPH mRNA in the brain stem shows that compared with the normal control group and 100 % dietary restricted group, the expressions of TPH mRNA in the brain stem have been enhanced significantly in 30 % dietary restricted group （ P〈0.05 ）. Compared with 100 % dietary restriction group, all of the dietary restriction groups have been enhanced significantly （P 〈0.05）. Conclusions：Dietary restriction enhances the metabolism of 5-HT in the brain stem of rats, which goes along with the different levels of dietary restricted.

关 键 词：饮食限制 中枢疲劳 5-羟色胺 色氨酸羟化酶 大鼠 动物实验 

分 类 号：G804.7[文化科学—运动人体科学]