荧光定量PCR在结核杆菌微菌落培养研究中的应用  

Application of Fluorescent Quantitative PCR in Research of Mycobacterium Tuberculosis Microcolony Culture

作　　者：余传星[1] 赵自云[2] 潘玉钦 朱玲[2] 

机构地区：[1]福建中医学院附属第二人民医院,福建福州350003 [2]福建医科大学基础免疫系及神经生物学研究中心,福建福州350004 [3]福州市晋安区疾病预防控制中心,福建福州350014

出　　处：《福建中医学院学报》2009年第6期51-53,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：卫生部科学研究基金--福建省卫生教育联合攻关项目(WKJ2005-2-007)

摘　　要：目的研究结核杆菌微菌落培养中细菌繁殖的周期。方法利用荧光定量PCR对微菌落形成前后细菌DNA的拷贝数进行检测分析,在基因水平判定结核杆菌生长繁殖一代所需要的时间以及观察微菌落形成的最短时间。结果对培养前后的拷贝数进行t检验,t值分别为4.35和3.97,ν=4,培养前后细菌数变化比较有显著差异(P<0.05)。结论结核杆菌培养12 h可以生长一代。Objective To study the reproductive cycle of bacteria in the culture of mycobacterium tuberculosis microcolony. Methods The DNA copy number of bacteria was detected before and after the formation of microcolony by fluorescent quantitative PCR to evaluate the tittle for mycobacterium tuberculosis to reproduce one generation and the shortest time of microcolony formation. Results The t test was performed in the copy number before and after the colony culture, and t values were 4.35 and 3.97, v=4, and the number of bacteria had significant difference before and after the colony culture （P〈0.05）. Conclusion Myeobacterium tuberculosis can reproduce one generation after cultivated for 12 hours.

关 键 词：结核杆菌 微菌落 培养 荧光定量PCR 

分 类 号：R378[医药卫生—病原生物学]