固定化离子液体吸附黄酮类化合物性能研究  

Adsorption Property of Supported Ionic Liquids for Flavonoids

作　　者：张娟娟[1] 曹树稳[1,2] 余燕影[2] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047 [2]南昌大学化学系,南昌330031

出　　处：《分析化学》2009年第12期1810-1814,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.20562008;20662008)资助项目

摘　　要：研究了吸附时间、固液比、样品浓度、上样流速对N-甲基咪唑键合硅胶固定化离子液体(SilprMim)吸附黄酮类化合物性能的影响。结果表明,受试化合物在30 min内均达到吸附平衡;吸附效率随固液比增大而增加,随样品浓度增大而降低;吸附等温线与拟合的Langmuir模型吻合良好。SilprMim对染料木素、木犀草素及槲皮素的饱和吸附量分别为47.7,52.5和63.2 mg/g,上样流速在0.5～1.5 mL/min范围内吸附效率达90%以上;甲醇洗脱染料木素、木犀草素及槲皮素的解吸率分别为86.1%,83.3%和84.6%;解吸顺序为:染料木素、木犀草素、槲皮素。SilprMim对3种受试黄酮类化合物具有较强吸附和分离能力,有望应用于黄酮类天然产物的分离纯化。The effects of adsorption time, solid-liquid ratio, sample concentration, flow rate on the adsorption properties of supported ionic liquids（the N-methylimidazolium functionalized silica, SilprMin） for flavonoids were investigated. The results indicated that the adsorption equilibrium for tested compounds, genistein, luteolin and quereetin, was achieved within 30 rain, the adsorption efficiencies of these three compounds were improved with the increase of solid-liquid ratio, and decreased with the increase of their concentrations. More-over, the adsorption isotherm data of the three tested compounds were in good concordance with the Langmuir model. The saturated adsorption capacity of SilprMim for genistein, luteolin and quercetin was 47.7, 52.5 and 63.2mg/g, respectively. Adsorption efficiency of SilprMim could reaeh more than 90% at a sample flow rate of 0.5 - 1.5 mL/min. Using methanol as eluent, the saturated desorption efficiencies of genistein, luteolin and quercetin were 86. 1% , 83.3% and 84.6% , respectively. The elution order of the three tested flavonoids was genistein, luteolin and quercetin. SilprMim had strong adsorption and separation capacity for three tested flavonoids, which was hopeful to be applied in separation and purification of naturally occurring flavonoids.

关 键 词：固定化离子液体 吸附 黄酮 

分 类 号：Q503[生物学—生物化学]