马来酸罗格列酮对去势大鼠骨微结构的影响  

Effect of rosiglitazone on bone microarchitecture of bilaterally ovariectomized rats

作　　者：陈胜广[1] 张倩[1] 盛春君[1] 程晓云[1] 杨篷[1] 曲伸[1] 吴国亭[1] 李文君[1] 

机构地区：[1]同济大学附属上海市第十人民医院内分泌科,上海200072

出　　处：《第二军医大学学报》2009年第12期1402-1405,共4页Academic Journal of Second Military Medical University

基　　金：上海市科委自然基金项目(07411963100)~~

摘　　要：目的:研究马来酸罗格列酮对去势大鼠骨微结构的影响。方法:21只24周龄Wistar大鼠双侧卵巢切除,随机分为对照组、罗格列酮A(RA)组、罗格列酮B(RB)组,每组7只。RA组、RB组每只大鼠分别给予马来酸罗格列酮0.35 mg/d、0.70 mg/d(以生理盐水溶解灌胃),对照组以相同体积生理盐水灌胃。给药4周后使用micro-CT分别测定股骨、胫骨、椎骨的骨量/组织量(BV/TV)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)、骨小梁分离度(Tb.Sp)、骨表面积/骨量(BS/BV)。结果:与对照组相比,RA组及RB组各部位(股骨、胫骨、椎骨)Tb.N、Tb.Th和BS/BV(RA组胫骨除外)均减少(P<0.05或P<0.01);胫骨、椎骨Tb.Sp增加(P<0.05),股骨Tb.Sp无改变(P>0.05);各部位BV/TV差异无统计学意义(P>0.05)。上述检测指标在RA组与RB之间差异无统计学意义(P>0.05)。结论:使用罗格列酮后,骨微结构受到损伤,且主要集中于松质骨。Objective：To study the effect of rosiglitazone on the bone microarchitecture of bilaterally ovariectomized（OVX） rats.Methods： Twenty-one 24-week-old skeletally mature rats were bilaterally ovariectomized,and were randomly divided into three groups（n=7）：Control group（C group）,Rosiglitazone A group（RA group）,and Rosiglitazone B group（RB group）.Rats in RA group were given i.g 0.35 mg/d rosiglitazone for four weeks and those in the RB group were given i.g 0.70 mg/d rosiglitazone.Rats in the control group were given normal saline in the same manner.The rats were sacrificed four weeks later.The bone volume/tissue volume（BV/TV）,the trabecular number（Tb.N）,the trabecular thickness（Tb.Th）,the trabecular separation（Th.Sp）,and the bone surface/bone volume（BS/BV）of the femur,tibia,and lumbar were determined by micro-computered tomography（micro-CT）.Results： Compared with the control group,the Tb.N,Tb.Th,and BS/BV of the femur,tibia,and lumbar all decreased significantly in RA group（except for the BS/BV of tibia）（P〈0.05,P〈0.01）;the Tb.SP of tibia and lumbar in the RA group were significantly increased（P〈0.05）;and there were no differences in BV/TV between the two groups.Compared with control group,the Tb.N,Tb.Th,and BS/BV of the femur,tibia,and lumbar were decreased significantly in RB group（P〈0.05,P〈0.01）;the Tb.Sp of tibia and lumbar increased significantly（P〈0.05）;and the BV/TV had no differences between the two groups.There were no significant differences between the RA group and RB group concerning all the parameters.Conclusion： Rosiglitazone can cause injury to the bone microarchitecture,especially to the cancellous part of the bone.

关 键 词：罗格列酮 卵巢切除术 骨 X线微型计算机断层扫描 

分 类 号：R588.6[医药卫生—内分泌]