白藜芦醇对小鼠胚胎成纤维细胞增殖和细胞周期的影响  

Resveratrol effects cell multiplication and cycle of Mouse embryonic fibroblast

作　　者：张黎[1] 李勇 张洁[1] 张秋红[3] 

机构地区：[1]广东药学院基础学院人体解剖学教研室,广东广州510006 [2]宁夏大学生命科学学院西部特色资源利用与保护教育部重点实验室,宁夏银川750021 [3]广东药学院门诊部,广东广州510006

出　　处：《细胞与分子免疫学杂志》2009年第12期1109-1111,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：广东省建设中医药强省科研课题资助项目(2009245)

摘　　要：目的:探讨白藜芦醇(Res)对小鼠胚胎成纤维细胞(MEF)增殖和细胞周期的影响及变化规律。方法:通过不同浓度(1～150μmol/L)Res作用于增殖期的MEF,用MTT法检测72h后各组细胞增殖变化,用流式细胞术检测并分析其细胞周期。结果:Res1μmo/L组A值明显高于对照组(P<0.05),Res50μmol/L和150μmol/L组A值分别为0.196±0.055和0.185±0.034,均明显低于对照组(分别P<0.05,P<0.01),其细胞生长抑制率分别为14.0%和18.9%。细胞周期检测结果表明,Res1μmol/L组中G2/M期细胞占(14.82±0.49)%显著高于对照组(P<0.01)。50μmol/L和150μmol/L组G0/G1期细胞所占比例分别为(59.61±6.03)%和(65.31±0.27)%均显著高于对照组(P<0.01)。结论:Res对MEF增殖的影响,具有双向浓度依赖性,增殖和抑制增殖均与细胞周期调控有关,其中阻断G1期细胞进入S期是其抑制MEF增殖主要因素。AIM： To discuss the cell multiplication and cell cycle situation in mouse embryonic fibroblast （ MEF）, which acted by Resveratrol （Res）. METHODS： Res with different concentration （0. 15 - 500 μmol/L） was used on MEF. After 72 h, cell multiplication situation and Inhibitory rate of cell growth were tested by MTT, and cell cycle was analyzed by using flow cytometry. RESULTS： A of the group with 1μmol/L Res was Significant higher than the control group （ P 〈 0.05 ）. A of groups with 50 μmol/L and 150 μmol/L were 0.196 ±0.055 and 0. 185 ± 0.034 separately, which were lower than the control group （ P 〈 0.05, P 〈 0.01 separately）, and the inhibitory rates of cell growth were 14. 0% and 18. 9% separately. Cell cycle analysis showed that the group with 1 μmol/L Res had （ 14.82 ± 0.49）% cells in G2/M period, which was higher than the control group （ P 〈 0.01 ）. The 50μmol/L and 150 μmol/L groups had （59.61±6.03）% and （65.31±0.27）% cells in G0/G1 period, those were higher than the control group （P〈0.01）. CONCLUSION： Two-way effect, which relies on the dose of Res, is showed on MEF. Multiplication and growth inhibition had relation with the control of cell cycle. Prevent cell turn into S period from G1 period in cell cycle is main inhibition growth effect in MEF.

关 键 词：白藜芦醇 成纤维细胞 细胞周期 

分 类 号：Q813.1[生物学—生物工程]