泰山赤灵芝多糖缓解大鼠动脉粥样硬化的分子机制研究  

Molecular mechanisms of Taishan Ganoderma Lucidum amylose in prevention of rat atherosclerosis

作　　者：王泽平[1] 顾洪雁[1] 伊淑莹[1] 

机构地区：[1]泰山医学院生物化学教研室,山东泰安271000

出　　处：《中国病理生理杂志》2009年第12期2310-2313,共4页Chinese Journal of Pathophysiology

基　　金：博士基金资助项目(No.2007B017)

摘　　要：目的:研究泰山赤灵芝多糖处理动脉粥样硬化大鼠前后血脂的变化和胸主动脉细胞差异表达的基因,了解泰山赤灵芝多糖治疗动脉粥样硬化的分子机制。方法:全自动生化分析仪测定泰山赤灵芝多糖处理动脉粥样硬化大鼠前后血清TG、TC、HDL-C含量的变化;通过差异显示逆转录PCR(DDRT-PCR)技术研究泰山赤灵芝多糖处理动脉粥样硬化大鼠前后胸主动脉细胞的基因表达差异,并用反向Northern证实差异显示基因片段。结果:泰山赤灵芝多糖处理动脉粥样硬化大鼠后,TG、TC含量均明显下降,而HDL-C和HDL-C/TC百分比明显升高;胸主动脉细胞出现一些表达上调和下调的基因片段。上调基因有细胞色素B561、ERP72、nitric oxide synthase 2和Gpx1;下调基因有胸腺素β4、FGFRI原癌基因伴随蛋白、FK506结合蛋白、ICAM-1、c-myc和TNF-α。结论:泰山赤灵芝多糖可减轻动脉粥样硬化风险,降低血脂。AIM： To study the change of blood lipid and difference of expressed gene in chest aorta cells of atherosclerosis model rats with or without the treatment of Taishan Ganoderma Lucidum amylose. METHODS ： The concentrations of triglyceride （TG）, total cholesterol （TC） and high-density lipoprotein cholesterol （HDL- C） in serum were measured by automatic serum biochemical assay. Differential - display reverse transcription - PCR （ DDRT - PCR） was used to analyze the differential expressed gene in chest aorta cells of atherosclerosis model rat with or without the treatment of Taishan Ganoderma Lucidum amylose. The differential expressed cDNA was confirmed by reverse Northern blotting. RESULTS ： Up - regulated and down - regulated cDNA were isolated in chest aorta cells of atherosclerosis model rats with or without Taishan Ganoderma Lucidum amylose treatment. The known up - regulated genes included cytB561, ERP72, nitric oxide synthase 2 and Gpxl. The known down - regulated genes included thymosin β4, FGFRI oncogene, ICAM - 1, FK506 binding protein, c -myc and TNF- α. CONCLUSION： Taishan Ganoderma Lucidum amylose decreases the level of blood lipid.

关 键 词：泰山赤灵芝 动脉粥样硬化 胸主动脉细胞 基因表达 

分 类 号：R543.5[医药卫生—心血管疾病]