缺氧调控滋养细胞和内皮细胞sFlt-1差异表达的研究  

Expression of soluble fms-like tyrosine kinase-1 under hypoxia in human first-trimester extravillous trophoblast cell line TEV-1 and human umbilical vein endothelial cell line EVC-304

作　　者：周琼[1] 刘海意[1] 曾万江[1] 龚洵[1] 吴媛媛[1] 乔福元[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《现代妇产科进展》2009年第11期844-846,共3页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨缺氧条件下,人早孕绒毛滋养细胞(TEV-1)及人脐静脉内皮细胞(EVC-304)中可溶性血管内皮生长因子受体-1(sFlt-1)差异表达的特点。方法:CoCl2诱导TEV-1及EVC-304化学缺氧,并于0,6,12,24,48,72,96,120h用ELISA法分别检测上清中sFlt-1蛋白表达,RealtimeRT-PCR法检测各组sFlt-1mRNA表达。结果:缺氧72h后滋养细胞sFlt-1mRNA及蛋白表达随时间延长明显升高(P<0.05),而内皮细胞sFlt-1mRNA及蛋白表达差异无统计学意义(P>0.05)。结论:正常情况下滋养细胞及脐静脉内皮细胞中均有sFlt-1表达;缺氧诱导滋养细胞sFlt-1表达明显增加,而内皮细胞sFlt-1表达对缺氧刺激无明显反应。Objective：To investigate the difference of sFlt-1 expression between human first-trimester extravillous trophoblast cell line TVE-1 and human umbilical vein endothelial cell line EVC-304 induced by chemical hypoxia with CoCl2. nethods：TEV-1 and EVC-304 were treated with CoCl2 for 0,6,12,24,48,72,96,120 hours. The protein levels of sFh-1 were detected respectively using ELISA, and the mRNA expressions of sFIt-1 were tested using realtime RT-PCR. Results： There was significant elevation of sFlt-1 expression in TEV-1 induced by CoCl2 after 72 hours （ P〈0.05 ）, but not in EVC-304 （ P〉0.05 ）. Conclusion： sFh-1 would be expressed both in TEV-1 and EVC-304,but only TVE-1 can express more sFlt-1 induced by hypoxia with CoCl2.

关 键 词：子痫前期 缺氧 SFLT-1 滋养细胞 脐静脉内皮细胞 

分 类 号：R714.245[医药卫生—妇产科学]