唐古特白刺组织培养及其培养基筛选研究  

Research on tissue culture and medium of Nitraria tangutorum

作　　者：郭晔红[1] 蔺海明[1] 武睿[1] 

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070

出　　处：《草业学报》2009年第6期59-64,共6页Acta Prataculturae Sinica

基　　金：甘肃省农牧厅农业生物技术专项(GNSW-2007-07);甘肃省教育厅项目(0702-13);甘肃省星火计划项目(5HS065-A91-001-06)资助

摘　　要：本试验选用唐古特白刺幼嫩茎段和叶片作为材料,研究白刺不同外植体的离体培养技术及其适宜的培养基。结果表明,白刺带芽嫩茎是诱导丛生芽的良好外植体,恒温20℃以下,可有效降低白刺丛生芽初代培养污染严重的程度;叶片是诱导愈伤组织的良好外植体,且低浓度生长素2,4-D培养基较高浓度培养基形成的白刺愈伤组织致密,也不易褐化。白刺的最适增殖、壮芽培养基为MS＋6-BA2 mg/L＋NAA1.00 mg/L,而且是以腋芽形成丛生芽的方式进行增殖的;最适生根培养基是1/2 MS＋KT 1 mg/L＋IBA0.5 mg/L;形成愈伤组织较好的培养基是MS＋2,4-D 0.5-1.0 mg/L。Young stem segments and leaves of Nitraria tangutorum were selected to investigate suitable media and plant regeneration techniques for explants.Immature stems were a good source for explants of tufted shoots,and cultivation at 20℃ can reduce contamination rates of tufted shoots.Leaves were good explants of callus induction as well and a lower concentration of 2,4-D was better for callus than a higher concentration.The optimum multiplication medium for N.tangutorum was MS＋6-BA 2 mg/L＋NAA 1.00 mg/L and tufted shoots formed from axil buds were the main multiplication method.The optimum medium for root formation was 1/2 MS＋KT 1 mg/L＋IBA 0.5 mg/L and the optimum medium for callus induction was MS＋2.4-D 0.5-1.0 mg/L.

关 键 词：唐古特白刺 组织培养 培养基 愈伤组织 

分 类 号：S567.19[农业科学—中草药栽培]