外源性金属硫蛋白对奶牛泌乳性能和抗氧化状况的影响  

Effects of exogenous metallothionein on milkability and anti-oxidative status in dairy cattle

作　　者：张彬[1] 谭琼[1] 肖兵南[2] 李丽立[3] 陈宇光[1] 肖定福[1] 刘海林[2] 吴宗明[1] 张复生[3] 

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]湖南省畜牧兽医研究所,湖南长沙410131 [3]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《草业学报》2009年第6期137-143,共7页Acta Prataculturae Sinica

基　　金：国家自然科学基金项目(3067151630170684);湖南省自然科学基金重点项目(06JJ20091);湖南省教育厅重点项目(08A029)资助

摘　　要：选取24头中国荷斯坦奶牛经产泌乳母牛,随机等分为A、B、C、D四组,分别每头静脉注射经生理盐水溶解的Zn-MT 0(对照),4.0,8.0和12.0 mg,以探讨外源性金属硫蛋白(MT)对奶牛泌乳性能和血清抗氧化状况的影响。结果表明,C和D组的4%标准乳产量均显著高于对照组(P<0.05),但各组间的乳脂率无显著差异(P>0.05)。B、C和D组血清谷胱甘肽过氧化物酶(GSH-Px)活性均显著高于对照组(P<0.05)。D组血清过氧化氢酶(CAT)活性分别显著高于B组(P<0.05)和对照组(P<0.01);C组血清CAT活性也显著高于对照组(P<0.05)。C和D组总抗氧化能力(T-AOC)分别显著高于对照组和B组(P<0.05)。B、C和D组血清丙二醛(MDA)含量均显著低于对照组(P<0.05)。说明MT不失为一种能够有效调控奶牛泌乳量和抗氧化能力的生理活性物质和高效抗氧化剂。The effects of exogenous metallothionein（Zn-MT） on milkability and anti-oxidative status of dairy cattle was studied using 24 lactating Chinese Holstein cows.They were randomly allocated to groups A,B,C,and D,and Zn-MT was supplemented at 0,4.0,8.0 and 12.0 mg/capita respectively by intravenous injection.The milk yields of the cows in groups C and D were higher（P〈0.05） than those in group A,but there were no significant differences in milk yields between groups A and B or in milkfat percentages between groups.The blood GSH-Px activities of the cows in groups B,C,and D were also higher（P〈0.05）than that in group A.The blood CAT activities of the cows in group D were higher than those in groups A（P〈0.01） and B（P〈0.05）,and there were significant differences（P〈0.05） in the blood CAT activities of group A and C cows.The T-AOC levels of the cows in groups C and D were also higher（P〈0.05） than those in groups A and B.The MDA serum content of cows in groups B,C,and D were lower（P〈0.05） than of cows in group A.Exogenous Zn-MT should be an effective anti-oxidative substance and physiological supplement for dairy cattle.

关 键 词：金属硫蛋白 泌乳性能 抗氧化 GSH-PX CAT T-AOC MDA 奶牛 

分 类 号：S823.91[农业科学—畜牧学]