风信子DFR基因全长cDNA的克隆及序列分析  

Cloning and Sequencing Full-length cDNA of DFR Gene in Hyacinthus

作　　者：祁银燕[1] 刘雅莉[1] 李莉[1] 王跃进[1] 

机构地区：[1]西北农林科技大学园艺学院农业部西北园艺植物种质资源利用重点开放实验室陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《中国农学通报》2009年第24期62-67,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"百合花色形成关键基因特异启动子功能与应用研究"(30871734)

摘　　要：以蓝色风信子完全露出蓝色的花蕾为材料,利用RT-PCR和RACE技术,获得风信子DFR基因的全长cDNA。该cDNA全长1252bp,开放阅读框为1098bp,编码366个氨基酸。Blast搜索结果显示,风信子DFR基因核苷酸序列与其它植物已报道的DFR基因具有66%～77%的相似性,氨基酸序列有62%～73%的相似性。聚类分析表明,最先与风信子聚类合并的是鸢尾,其次与其它单子叶植物聚类,最后与双子叶植物聚类。A total length DFR gene was cloned with RT-PCR and RACE technology from flower bud with blue petals of Hyacinthus orientalis L.. Hyacinthus DFR cDNA was 1252 bp in length. Within the full-length cDNA, a clear open reading frame （ORF） was 1098 nucleotides, coding 366 amino acids. The results of homologous analysis in GenBank demonstrated that the sequence had 66 %-77 % identity on the nucleotide sequence and 62 %-73% identity on the deduced amino acid sequence. The results of phylogenetie analysis showed that DFR from Hyacinthus orientalis and from Iris x hollandica clustered together firstly, and then came those from other monocotyledon, dicotyledon came subsequently.

关 键 词：DFR CDNA克隆 风信子 基因 

分 类 号：S3[农业科学—农艺学]