怀山药根际土壤微生物、酶活性和酚酸物质变化及其关系研究  

Study on the Change of Microbial Quantity, Enzyme Activity And Phenolic Acids in Rehmannia glutinosa Rhizosphere Soil

作　　者：乔卿梅[1] 程茂高[1] 王新民[1] 魏志华[1] 

机构地区：[1]郑州牧业工程高等专科学校药物工程系,郑州450011

出　　处：《中国农学通报》2009年第24期151-154,共4页Chinese Agricultural Science Bulletin

基　　金：河南杰出青年科学基金"河南省道地(地产)药材GAP栽培与质量控制研究"(074100510018);国家第四十一批博士后科学基金"怀牛膝道地性成因的土壤微域环境研究"(20070410616)

摘　　要：对不同生育期怀山药根际土壤微生态变化及其关系进行研究,为研究怀山药根际微生态与其品质的相关性奠定基础。采用室内测定法研究怀山药根际土壤微生物数量、土壤酶活性及酚酸物质含量。结果表明:怀山药在其生长发育过程中,根际土壤中酚酸物质的含量和土壤细菌、放线菌、真菌数量均呈显著升高趋势,且根茎膨大速度越快时上升趋势越明显;根际细菌绝对数量一直占根际土壤微生物的80%左右,并呈增长趋势;过氧化氢酶、多酚氧化酶、蛋白酶活性逐渐增强,蔗糖酶、磷酸酶、脲酶活性呈下降趋势;根际土壤中酚酸物质的含量与根际土壤中微生物的含量和多数土壤酶活性呈显著相关性。怀山药在其生长发育过程中根际微生态发生了显著变化,土壤中酚酸物质、微生物和酶活性间存在密切关系。This paper studied the variations of phenolic acids contents, microbes flora and enzyme activity in yam Rehmannia glutinosa rhizosphere soil and their relations. The results showed that the content of phenolic acids and the numbers of fungi, bacteria, actinomycetes had a prominent increase with the yam growing development. The mone fast of rootstalk growing rate, the mone distinct of increase tendency. While the number of bacteria in yam rhizosphere soil were about 80% all the time, and showed increase tendency. The enzyme activity of catalase, polyphenol oxidase, protease in rihzosphere showed increase tendency, and the variation of enzyme activity in sucrase, phosphatase, urease were contrary. The content of rhizosphere phenolic acids presented a prominent pertinence with the numbers of fungi, bacteria, actinomycetes and the enzyme activity of catalase, polyphenol oxidase, protease, phosphatase, urease in rhizosphere soil. This means rhizosphere microecological had a prominent change during the yam growing development, and it had close relation between phenolic acid, microorganism and enzyme activity in rhizosphere soil.

关 键 词：怀山药 根际微生物 土壤酶活性 酚酸 

分 类 号：S154.37[农业科学—土壤学] S567.9[农业科学—农业基础科学]