抗碱性成纤维细胞生长因子单克隆抗体的制备及特异性分析  

PREPARATION AND SPECIALITY ANALYSIS OF HYBRIDOMA SECRETING MONOCLOAL ANTIBODIES TO BASIC FIBROBLAST GROWTH FACTOR

作　　者：向军俭[1,2] 刘小青[1,2] 廖俊珊 林剑[1,2] 

机构地区：[1]暨南大学生物工程学系 [2]暨南大学生物工程研究所

出　　处：《中国免疫学杂志》1998年第5期371-374,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金

摘　　要：以纯化的重组碱性成纤维细胞生长因子为抗原，免疫小鼠取其脾细胞与ＳＰ２／０细胞的融合，获得了多株稳定分泌抗碱性成纤维细胞生长因子单克隆抗体杂交瘤细胞株；并以Ｗｅｓｔｅｒｎｂｌｏｔ和单克隆抗体抑制碱性成纤维细胞生长因子促进ＢＡＬＢ／Ｃ３Ｔ３细胞生长作用以及ＡＢＣ免疫组化分析了抗体的特异性。Purfied recombinant basic fibroblast growth factor(bFGF) expressed in Escherichia coli was applied as antigen to mice.Humman basic fibroblast growth factor antiserum from mousc was prepared.What is more,several hybridoma cells secreting monoclonal antibodics to basic fibroblast growth factor were obtained via fusion of immuned mouse spleen cells and Sp2/O cells.It is fact that the monoclonal antibodies react spccially to basic fibroblast growth factor by ELISA,inhibition of hbFGF activity and ABC immunohistochemisty.

关 键 词：BFGF 单克隆抗体 制备 特异性分析 

分 类 号：R392－33[医药卫生—免疫学]