环境因子对眼斑拟石首鱼精子活力的影响  

Effects of environmental factors on the vitality of sperm in csiaenops ocellatus

作　　者：魏平[1] 叶霆[1] 吴向丹[2] 闫家强[1] 竺俊全[1] 

机构地区：[1]宁波大学教育部应用海洋生物技术重点实验室,宁波315211 [2]宁波大学图书馆,宁波315211

出　　处：《生态科学》2009年第5期438-442,共5页Ecological Science

基　　金：宁波市科学技术局项目(2006C100044;2007A31004);教育部创新团队项目(IRTO734)

摘　　要：通过测定精子的激活率、运动时间及寿命,研究了pH、盐度、离子及葡萄糖等因子变化对眼斑拟石首鱼(Csiaenops ocellatus)精子活力的影响。结果表明,眼斑拟石首鱼精液中精子平均浓度为(1.203±0.22)×10^(10)个·mL^(-1);精子激活与运动的适宜盐度范围为20～35,其中盐度25时精子的激活率、运动时间及寿命分别达94.32%、9.14min及12.55min;精子激活与运动的适宜pH为6.0～8.5,适宜的KCl、NaCl及CaCl_2溶液浓度分别为600～700 mmol·L^(-1)、600mmol·L^(-1)及400～600 mmol·L^(-1),适宜的葡萄糖溶液浓度为700～900mmol·L^(-1);精子在缺少HCO_3^-或Mg^(2+)或Ca^(2+)的人工海水中激活率与在人工海水中的激活率无显著差异,但运动时间和寿命有所缩短。The effects of pH value, salinity, glucose and ions solutions on the sperm vitality of Csiaenops ocellatus were investigated by measuring the activation ration, moving time and life-span of sperm. The results indicated that the average density of sperm was （1.203±0.22） ×10^10·ml^-1 and the suitable salinity for sperm activation and movement was 20-35, while pH value 6.0-8.5. The activation rate, moving time and life-ban of sperm reached to 94.32%, 9.14 min and 12.55 min respectively when the sperm was activated in salinity 25. The proper concentration of KCl, NaCl, CaCl2 solutions for sperm activation and movement was 600-700 mmol·L-1,600 mmol·L^-1 and 400-600 mmol·L^-1 respectively, while the proper concentration of glucose solution was 700-900mmol·L^-1. No significant differences were found in the activation rate when the sperm was activated by the artificial seawater lacking of HCO3^-,Mg^2＋or Ca^2＋, but the moving lime and survival time of sperm became shorter.

关 键 词：眼斑拟石首鱼 精子 活力 环境因子 

分 类 号：S965.399[农业科学—水产养殖]