汩罗江与柳江鳤鱼线粒体细胞色素b基因的遗传变异初探  

Preliminary study on genetic variation of mitochondrial DNA cytochrome b gene of Ochetobius elogatus in Miluojiang River and Liujiang River

作　　者：范凤娟[1] 章群[1] 

机构地区：[1]暨南大学水生生物研究所,广州510632

出　　处：《生态科学》2009年第5期457-459,共3页Ecological Science

基　　金：国家自然科学基金(30770415);留学归国人员科研启动基金(2005)资助项目

摘　　要：测定并分析了长江水系汨罗江4尾鳤鱼(Ochetobius elongates)和珠江水系柳江2尾鳤鱼的线粒体细胞色素b基因部分序列981bp,发现6个单倍型,其中19个变异位点,简约信息位点15个;AT含量和GC含量分别为57.6%和42.4%。在基于Kimura双参数模型的邻接树上,汩罗江和柳江鳤鱼各自聚群。汨罗江4尾鳤鱼间的遗传距离为0.001～0.007,柳江2尾鳤鱼间遗传距离为0.001,而2群体间的平均遗传距离为0.015(0.013～0.017),群体间的遗传变异大于群体内的遗传变异,表明汩罗江鳤鱼与柳江鳤鱼出现较明显的遗传分化。由于本研究采集地理群体少,分析的样本小,为更好地揭示长江和珠江水系鳤鱼的遗传变异,上述结论尚需进一步分析更多大样本的地理群体加以验证。Partial mitochondrial DNA Cytochrome b gene in Ochetobius elogatus from Miluojiang River and Liujiang River was sequenced and analyzed in present study. Six haplotypes were determined, of which 19 variable sites and 15 parsimony-informatic sites were found. AT content and GC content were 57.6% and 42.4%, respectively. On the neighbor-joining tree based on Kimura-2 parameter model, individuals from Miluojiang River and Liujiang River were clustered separately. Genetic variation among 4 individuals from Miluojiang population was 0.001-0.007, and that between 2 individual of Liujiang population was 0.001. Genetic variation between 2 populations ranged from 0.013 to 0.017 with an average of 0.015. The results indicated that genetic variation was much greater between populations than within populations, and genetic differentiation was obvious between Mihiojiang population and Liujiang population. However, the conclusion about genetic variation in the two rivers should be further validated by collecting more samples from more locations.

关 键 词：鳍鱼 细胞色素B 遗传变异 

分 类 号：Q178.532[生物学—水生生物学]