低频超声波和甘油对皮肤组织光学透明的反射光谱研究  

Reflectance Spectroscopy Study of Low-Frequency Ultrasound and Glycerol on Skin Optical Clearing

作　　者：钟会清[1] 郭周义[1] 魏华江[1] 张祖德[1] 杨洪钦[1] 谢树森[2] 

机构地区：[1]华南师范大学激光生命科学教育部重点实验室,广东广州510631 [2]福建师范大学医学光电科学与技术教育部重点实验室,福建福州350007

出　　处：《光谱学与光谱分析》2009年第12期3190-3194,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60778047);广东省自然科学基金项目(06025080,0632270);医学光电科学与技术教育部重点实验室(福建师范大学)开放课题项目(JYG0804,JYG0503)资助

摘　　要：研究了经低频超声波和促渗剂处理后的猪皮组织在波长为400～860nm范围内随时间变化反射光谱特性的变化及各组于580nm处分别在0～15min和15～30min时间段各组反射光谱的改变程度。实验采用带积分球附件的AvaSpec-2048光纤光谱仪测量系统。结果表明:在波长为400～860nm范围内,空白对照组在整个过程中组织的反射光谱变化比较少;经超声波处理后组织对光的反射率在0～15min内是增加的,而且变化相对明显,但随着距超声波停止处理的时间加长,组织对光的反射率又慢慢恢复原状;经80%甘油处理组相对于空白对照组反射率减少得比较快;经超声波和80%甘油处理后组织的反射率变化最明显,特别是在0～15min内下降速度最快,而且对于波长580nm处在0～15min时间内第4组组织反射率的变化的速率与其他组在相同时间段内比较是:为第1组的约4.0倍和第3组的约2.3倍(在0～15min时间内),第1组组织反射率减少了1.896%;第3组组织反射率减少了3.316%;第4组组织反射率减少了7.551%。从上面的结果发现,超声波和促渗剂80%甘油对皮组织的光透明性不仅具有协同效应,而且可以使皮组织在短时间改善光透明性。In order to find a non-invasive way to improve the efficacy of skin optical clearing with topically applied optical clearing agents,the authors researched the changes of reflectance spectroscopy of skin tissues before and after being dealt by low-frequency ultrasound and osmotic active chemical agents within the wavelength range of 400-860 nm,and the degree of changes in reflectance spectroscopy of each group skin during 0-15 min and 15-30 min at 580 nm.The measurements were performed using a AvaSpec-2048 optical fiber spectrometer with integrating-sphere setup.The results of measurements showed that there were a few changes of the reflectance spectroscopy of skin tissues in the control group（Group 1）（The skin tissue was dealt with nothing.）during the whole observation;But in the Group 2（The skin tissue was dealt with only low-frequency ultrasound）,it was found that the reflectance of the skin tissue showed a significant increase comparing 15 min with 0 min,but the changes in reflectance of the tissues slowly restored the original form following the longer time since the ultrasound stopping;There was also a very fast changes in reflectance of the skins in the Group 3（the skin were dealt with only 80% glycerol）compared with that in the Group 1.The authors also found that there was a very distinct decrease in the reflectance of skin tissues dealt with both low-frequency ultrasound and 80% glycerol group（Group 4）,especially during the 0-15 min,and its speed was 4.0 times that of the Group 1 and 2.3 times that of Group 3（During 0-15 min,the reflectance of skin tissues in the Group 1 decreased 1.896%;the reflectance of skin tissue in the Group 3 decreased 3.316%;the reflectance of skin tissues in the Group 4 decreased 7.551%）.From the above results,it can be clearly seen that the low-frequency ultrasound and 80% glycerol not only have synergistic effect on optical clearing of skin tissue in vitro,but can change the optical clearing of the skin tissue in a short time.

关 键 词：光学透明 甘油 低频超声波 反射光谱 生物组织 

分 类 号：R318.5[医药卫生—生物医学工程]