胆管癌新鲜离体组织的傅里叶变换红外光谱研究  

Investigation on Fourier Transform Infrared Spectrum of Freshly Resected Cholangiocarcinoma Tissue

作　　者：尹刚[1] 徐智[1] 徐怡庄[2] 孙启刚[1] 刘亚奇[1] 张元福[2] 吴瑾光[2] 周孝思[1] 凌晓锋[1] 

机构地区：[1]北京大学第三医院普通外科,北京100191 [2]北京大学化学与分子工程学院,北京100871

出　　处：《光谱学与光谱分析》2009年第12期3241-3245,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50673005);北京市自然科学基金项目(2072020)资助

摘　　要：该研究探讨了运用傅里叶变换红外(FTIR)光谱技术对胆管癌进行术中原位、无创和快速诊断的可行性。联合应用傅里叶变换红外光谱仪和衰减全反射(ATR)探头,测定了26例胆管癌和43例良性胆管疾病新鲜离体组织的红外吸收光谱,发现每条光谱在3800～1000cm-1之间循序出现了12个谱带。测量各个谱带的峰位、峰强和峰面积值,然后计算出各谱带的相对峰强和相对峰面积比值,最后进行标准统计学分析。比较胆管癌与良性胆管组织的光谱发现有以下特征:(1)2925cm-1谱带明显地向低波数移位(P=0.033);(2)峰强比I1083/I1460(P=0.005)和峰面积比S1083/S1460(P=0.001),S1240/S1460(P=0.025)明显升高,说明癌组织中核酸相对脂类含量明显增加;(3)峰强比I1550/I1083(P=0.000)和峰面积比S1550/S1083(P=0.000)明显下降,提示蛋白质相对核酸的含量下降。研究结果表明,FTIR有望成为一种术中原位、在体和快速诊断胆管癌的新方法。To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of cholangiocarcinoma by Fourier transform infrared（FTIR）spectroscopy,26 samples of freshly resected cholangiocarcinoma tissues and 43 samples of benign bile duct tissue were collected during surgery.And all fresh samples were measured by FTIR spectroscopy via probe of attenuated total reflection（ATR）without pretreatment immediately after resection.For each spectrum,12 bands were identified and assigned between 3 800 and 1 000 cm-1.The peak position was found,and the intensity and area of the bands were measured;The ratios of the intensity and area were calculated.Standard statistic analysis was performed.The results illustrated that the FTIR spectra of malignant tissue were significantly different from those of benign tissue：1.The bands related to lipid：The peak of 2 925 cm-1 shifted to lower wave number significantly（P=0.033）;2.The bands related to nucleic acid and lipids：the ratios of I1 083/I1 460（P=0.005）,S1 083/S1 460（P=0.001）and S1 240/S1 460（P=0.025）raised significantly,indicating that the relative quantity of nucleic acid to lipids had evidently increased in malignancy.3.The bands related to protein：The ratios of I1 550/I1 083（P=0.000）and S1 550/S1 083（P=0.000）reduced significantly,implying that the relative quantity of protein to nucleic acid in malignancy decreased significantly and the secondary structure of protein changed probably.Our primary result illustrated that the FTIR spectroscopy technique maybe a promising method for in situ and quick intraoperative diagnosis of cholangiocarcinoma and has great value for clinical application.

关 键 词：FTIR 胆管癌 新鲜离体组织 

分 类 号：O657.3[理学—分析化学]