HIV/AIDS病人肝脏HIV-1 RNA和p24抗原的检测  

Detection of HIV-1 RNA and p24 antigen in HIV1-infected human liver

作　　者：赵鹏[1] 赵敏[1] 吉英杰[1] 姜天俊[1] 聂为民[1] 秦恩强[1] 赵景民[1] 周光德[1] 谢杨新[1] 张新伟[1] 张昕[1] 

机构地区：[1]解放军第302医院,北京100039

出　　处：《中国艾滋病性病》2009年第6期575-577,共3页Chinese Journal of Aids & STD

基　　金：首都医学发展科研基金项目(2007-3036)

摘　　要：目的了解艾滋病病毒Ⅰ型(HIV-1)在肝组织的表达和分布情况,探讨HIV/艾滋病(AIDS)病人发生肝损害的可能机制。方法先后采用免疫组织化学方法和核酸原位杂交方法,检测HIV/AIDS病人肝组织病理切片中的HIV-p24抗原和HIV-1核酸。结果14例HIV/AIDS病人肝切片组织的Kupffer细胞、淋巴细胞、血管内皮细胞和肝细胞,均检测到HIV-p24抗原。所有切片组织的Kupffer细胞、淋巴细胞有HIV RNA阳性表达,其中4例标本的肝细胞浆内有HIV RNA表达。结论HIV-1可能在肝脏实质细胞和间质细胞内复制表达,并引起肝脏损害。Objective To detect HIV-1RNA and p24 antigen in the liver biopsy specimens of people living with HIV/AIDS. Methods The p24 antigen and HIV-1RNA of liver biopsy samples of 14 patients with HIV/AIDS were examined by immunohistochemistry and in situ hybridization, respectively. Result Intracellular expression of p24 antigen was detected in Kupffer cells,lymphocytes, endothelial cells and hepatocytes of all specimens. In the in situ hybridization test, Kupffer cells and lymphocytes were labeled in all specimens. Furthermore, in 4 specimens the morphology of the HIVRNA positive cells was reproducidly consistent with that of hepatocytes. Conclusion These findings indicate that HIV-1 could replicate in the liver of HIV infected individuals and might be related to the damaged liver cells .

关 键 词：艾滋病病毒 肝组织 免疫组织化学 核酸原位杂交 

分 类 号：R373.1[医药卫生—病原生物学]