亚砷酸钠对Chang肝细胞鸟苷三磷酸环化水解酶及6-丙酮酰四氢蝶呤合成酶的mRNA表达水平的影响  

Effects of Sodium Arsenite on mRNA Expression of GTP Cyclohydrolase and 6-pyruvoyl-tetrahydropterin Synthase in Chang Liver Cells

作　　者：李昕[1] 侯萍[2] 李冰[1] 张新玉[1] 朱博[1] 徐苑苑[1] 孙贵范[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生教研室,辽宁沈阳110001 [2]中国医科大学91期七年制学生,辽宁沈阳110001

出　　处：《环境与健康杂志》2009年第12期1051-1053,共3页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(30400362)

摘　　要：目的研究亚砷酸钠(NaAsO2)对Chang肝细胞鸟苷三磷酸环化水解酶I型(GTPCH)及6-丙酮酰四氢蝶呤合成酶(PTPS)的mRNA表达水平的影响。方法分别以不同浓度NaAsO2(0、50、200及400μmol/L)染毒人类Chang肝细胞株12h。以MTT法测定细胞活力,以RT-PCR法测定GTPCH和PTPS mRNA的表达水平。结果50μmol/L剂量组细胞活力与对照组相比差异无统计学意义(P>0.05),其他两组细胞活力均显著低于对照组(P<0.05);50、200及400μmol/L剂量组GTPCH和PTPS的mRNA表达水平与对照组相比均显著下降(P<0.05),且呈剂量-效应关系。结论NaAsO2暴露引起Chang肝细胞四氢生物蝶呤(BH4)合成酶GTPCH和PTPS的mRNA表达水平下降可能与砷致色素代谢异常有关。Objective To study the effects of sodium arsenite on the mRNA expression of GTP cyclohydmlase （GTPCH） and 6-pyruvoyl-tetrahydropterin synthase （PTPS） in Chang liver cells. Methods Human Chang liver cells were cultured with sodium arsenite at doses of 0, 50, 200 and 400 μmol/L for 12 hours. Cell viability was tested by MTr assay, and the expression of mRNA of GTPCH and PTPS was detected by RT-PCR. Results Cell viability in 50 μmol/L group was almost the same as that in control, while the viability in the other two groups were significantly lower than that in control. All of the mRNA expression levels in the three groups were significantly lower than that in control with dose-dependent manner. Conclusion The down-regulated expression of GTPCH and PIPS, the main enzymes of the synthesis of BH4, induced by sodium arsenite exposure may be related with arsenic related skin depigmentation.

关 键 词：砷 亚砷酸钠 BH4 鸟苷三磷酸环化水解酶Ⅰ型 6-丙酮酰四氢蝶呤合成酶 

分 类 号：R994.6[医药卫生—毒理学]