白藜芦醇衍生物在大鼠尿液中代谢产物结构及代谢途径研究  

Studies on metabolic structures and pathways of resveratrol derivative in rat urine

作　　者：马宁[1,2] 李娟[2] 王建芬[2] 徐芳[2] 肖海英[2] 杨春华[3] 徐康平[4] 向大雄[2] 

机构地区：[1]中南大学湘雅二医院临床药学研究室,长沙410011 [2]长沙医学院,长沙410219 [3]药物质量与安全预警教育部重点实验室,中国药科大学药物分析教研室,南京210009 [4]中南大学药学院,长沙410013

出　　处：《药物分析杂志》2009年第12期1994-2001,共8页Chinese Journal of Pharmaceutical Analysis

基　　金："重大新药创制"科技重大专项(2009ZX09103-012

摘　　要：目的:研究大鼠尿液中白藜芦醇衍生物(E)-3,5,4′-三甲氧基-1,2-二苯乙烯(BTM-0512)的主要代谢产物结构及代谢途径。方法:尿样采用C18固相萃取小柱(SPE)处理,用LC/MS/MS技术分析推测BTM-0512在大鼠体内代谢产物的结构,结合UV、IR、NMR等技术对代谢产物M1进行结构确证。结果:BTM-0512在大鼠体内有多种代谢物,所建立的LC/MS/MS方法可用于寻找并鉴定主要代谢产物。结论:首次从大鼠尿液中鉴定出3个Ⅰ相和1个Ⅱ相代谢产物(M1-M4),推断出BTM-0512在大鼠体内的代谢途径,并确证了代谢产物M1的结构。Objective：To identify the major metabolites and metabolic pathways of resveratrol derivative （E）-3,5,4＇-trimethoxystilbene,BTM-0512） in rat urine.Methods：Samples were pretreated by using solid-phase extraction technique （SPE） with C18 cartridges,and a LC/MS/MS method with electrospray ionization （ESI）,positive ion mode and collision induced dissociation （CID）,was used to elucidate the structures of the major metabolites of BTM-0512.UV,IR and NMR were used to identify the structures of M1. Results：The results proved that the LC/MS/MS method established was simple,reliable and sensitive,and can be easily screened and identified the structures of the major metabolites of BTM-0512 in rat urine;it was better to understand its in vivo metabolism.Conclusion：The main metabolites of BTM-0512 are three phase Ⅰand one phase Ⅱmetabolites in rat urine,the possible metabolic pathways is proposed for the first time.The structure of M1 is authenticated by combining with spectra technologies.

关 键 词：(E)-3 5 4′-三甲氧基-1 2-二苯乙烯 代谢物 液相色谱-串联质谱 

分 类 号：R917[医药卫生—药物分析学]