高效液相色谱法测定茅苍术药材中β-桉叶醇与苍术素的含量  

HPLC determination of β-eudesmol and atractylon in Atractylodes lancea (Thunb.) DC.

作　　者：张晓兰[1] 李锦[1] 李遇伯[1] 王晓玉[1] 张丽[1] 顾明山[1] 

机构地区：[1]天津中医药大学中药学院,天津市中药化学与分析重点实验室,天津300193

出　　处：《药物分析杂志》2009年第12期2051-2054,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金资助项目(30801540)

摘　　要：目的:建立茅苍术药材中β-桉叶醇和苍术素含量的测定方法。方法:采用高效液相色谱法,色谱柱为Comatex C18-AB(150mm×4.6mm,5μm)柱,流动相为乙腈-水-碳酸氢铵(65∶35∶0.2),流速1.0mL·min-1,检测波长203nm,柱温为室温(25℃)。结果:被测定峰与相邻峰可达到基线分离;β-桉叶醇和苍术素的线性范围分别为2.3～115.0μg·mL-1(r=1.000)和1.12～56.0μg·mL-1(r=0.9999);平均回收率(n=9)分别为99.3%和99.9%。结论:本方法准确、简便,重复性好,可作为茅苍术中β-桉叶醇和苍术素的含量测定方法。Objective：To develop an RP-HPLC method for simultaneous determination of β-eudesmol and atractylon in Atractylodes lancea.Methods：The determination was performed by HPLC method,a Comatex C18-AB column（150 mm×4.6 mm,5 μm） was adopted with a mobile phase of acetonitrile-water-ammonium bicarbonate（65∶35∶0.2） at a flow rate of 1.0 mL·min-1;The detection wavelength was set at 203 nm and the column temperature was 25 ℃.Results：The linear ranges of β-eudesmol and atractylon were 2.3-115.0 μg·mL-1（r=1.000） and 1.12-56.0 μg·mL-1（r=0.9999）,respectively;The average recoveries（n=9） of the two components in Atractylodes lancea were 99.3% and 99.9%,respectively.Conclusion：The method is simple,precise and reliable for determination of β-eudesmol and atractylon in Atractylodes lancea （Thunb.） DC.

关 键 词：茅苍术 Β-桉叶醇 苍术素 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]